Overview

  • Product name
  • Description
    Rabbit polyclonal to SDHD
  • Specificity
    ab189945 will detect three isoforms of SDHD.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SDHD (internal sequence). The exact sequence is proprietary. NCBI Accession No. NP_002993.1. The synthetic peptide corresponds to 15 amino acids.
    Database link: O14521

  • Positive control
    • Human kidney tissue; mouse EL4 cell lysate and cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab189945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 17 kDa.
IHC-P Use a concentration of 2.5 µg/ml.

Target

  • Function
    Membrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
  • Pathway
    Carbohydrate metabolism; tricarboxylic acid cycle.
  • Involvement in disease
    Defects in SDHD are a cause of hereditary paragangliomas type 1 (PGL1) [MIM:168000]; also known as familial non-chromaffin paragangliomas type 1. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL1 is a rare autosomal dominant disorder which is characterized by the development of mostly benign, highly vascular, slowly growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors. Penetrance of PGL1 is incomplete when the disease is transmitted through fathers. No disease phenotype is transmitted maternally.
    Defects in SDHD are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
    Defects in SDHD may be a cause of susceptibility to intestinal carcinoid tumor (ICT) [MIM:114900]. A yellow, well-differentiated, circumscribed tumor that arises from enterochromaffin cells in the small intestine or, less frequently, in other parts of the gastrointestinal tract.
    Defects in SDHD are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
    Defects in SDHD are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus.
  • Sequence similarities
    Belongs to the CybS family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBT1 antibody
    • CII 4 antibody
    • CII-4 antibody
    • CII4 antibody
    • CWS3 antibody
    • CybS antibody
    • DHSD_HUMAN antibody
    • mitochondrial antibody
    • OTTHUMP00000234720 antibody
    • OTTHUMP00000234721 antibody
    • OTTHUMP00000234722 antibody
    • OTTHUMP00000234723 antibody
    • OTTHUMP00000234724 antibody
    • OTTHUMP00000234725 antibody
    • OTTHUMP00000234726 antibody
    • PGL antibody
    • PGL1 antibody
    • QPs3 antibody
    • SDH4 antibody
    • sdhD antibody
    • Succinate dehydrogenase [ubiquinone] cytochrome b small subunit antibody
    • Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial antibody
    • Succinate dehydrogenase complex subunit D antibody
    • Succinate dehydrogenase complex, subunit D, integral membrane protein antibody
    • Succinate dehydrogenase ubiquinone cytochrome B small subunit antibody
    • Succinate ubiquinone oxidoreductase cytochrome b small subunit antibody
    • Succinate ubiquinone reductase membrane anchor subunit antibody
    • Succinate-ubiquinone oxidoreductase cytochrome b small subunit antibody
    • Succinate-ubiquinone reductase membrane anchor subunit antibody
    see all

Images

  • Lane 1 : Anti-SDHD antibody (ab189945) at 1 µg/ml
    Lane 2 : Anti-SDHD antibody (ab189945) at 2 µg/ml

    Lane 1 : mouse EL4 cell lysate
    Lane 2 : mouse EL4 cell lysate


    Predicted band size : 17 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue, labeling SDHD using ab189945 at 2.5 µg/ml.

References

This product has been referenced in:
  • Jiang Q  et al. A novel germline mutation in SDHA identified in a rare case of gastrointestinal stromal tumor complicated with renal cell carcinoma. Int J Clin Exp Pathol 8:12188-97 (2015). Read more (PubMed: 26722403) »

See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab189945.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up