Overview

  • Product nameAnti-SDHD antibody
    See all SDHD primary antibodies
  • Description
    Mouse monoclonal to SDHD
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 57-161 of Human SDHD with a tag of 26 kDa. Total MW of immunogen is 38 kDa.

Properties

Applications

Our Abpromise guarantee covers the use of ab76607 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 17 kDa).

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMembrane-anchoring subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q).
    • PathwayCarbohydrate metabolism; tricarboxylic acid cycle.
    • Involvement in diseaseDefects in SDHD are a cause of hereditary paragangliomas type 1 (PGL1) [MIM:168000]; also known as familial non-chromaffin paragangliomas type 1. Paragangliomas refer to rare and mostly benign tumors that arise from any component of the neuroendocrine system. PGL1 is a rare autosomal dominant disorder which is characterized by the development of mostly benign, highly vascular, slowly growing tumors in the head and neck. In the head and neck region, the carotid body is the largest of all paraganglia and is also the most common site of the tumors. Penetrance of PGL1 is incomplete when the disease is transmitted through fathers. No disease phenotype is transmitted maternally.
      Defects in SDHD are a cause of susceptibility to pheochromocytoma (PCC) [MIM:171300]. A catecholamine-producing tumor of chromaffin tissue of the adrenal medulla or sympathetic paraganglia. The cardinal symptom, reflecting the increased secretion of epinephrine and norepinephrine, is hypertension, which may be persistent or intermittent.
      Defects in SDHD may be a cause of susceptibility to intestinal carcinoid tumor (ICT) [MIM:114900]. A yellow, well-differentiated, circumscribed tumor that arises from enterochromaffin cells in the small intestine or, less frequently, in other parts of the gastrointestinal tract.
      Defects in SDHD are a cause of paraganglioma and gastric stromal sarcoma (PGGSS) [MIM:606864]; also called Carney-Stratakis syndrome. Gastrointestinal stromal tumors may be sporadic or inherited in an autosomal dominant manner, alone or as a component of a syndrome associated with other tumors, such as in the context of neurofibromatosis type 1 (NF1). Patients have both gastrointestinal stromal tumors and paragangliomas. Susceptibility to the tumors was inherited in an apparently autosomal dominant manner, with incomplete penetrance.
      Defects in SDHD are a cause of Cowden-like syndrome (CWDLS) [MIM:612359]. Cowden-like syndrome is a cancer predisposition syndrome associated with elevated risk for tumors of the breast, thyroid, kidney and uterus.
    • Sequence similaritiesBelongs to the CybS family.
    • Cellular localizationMitochondrion inner membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CBT1 antibody
      • CII 4 antibody
      • CII-4 antibody
      • CII4 antibody
      • CWS3 antibody
      • CybS antibody
      • DHSD_HUMAN antibody
      • mitochondrial antibody
      • OTTHUMP00000234720 antibody
      • OTTHUMP00000234721 antibody
      • OTTHUMP00000234722 antibody
      • OTTHUMP00000234723 antibody
      • OTTHUMP00000234724 antibody
      • OTTHUMP00000234725 antibody
      • OTTHUMP00000234726 antibody
      • PGL antibody
      • PGL1 antibody
      • QPs3 antibody
      • SDH4 antibody
      • sdhD antibody
      • Succinate dehydrogenase [ubiquinone] cytochrome b small subunit antibody
      • Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial antibody
      • Succinate dehydrogenase complex subunit D antibody
      • Succinate dehydrogenase complex, subunit D, integral membrane protein antibody
      • Succinate dehydrogenase ubiquinone cytochrome B small subunit antibody
      • Succinate ubiquinone oxidoreductase cytochrome b small subunit antibody
      • Succinate ubiquinone reductase membrane anchor subunit antibody
      • Succinate-ubiquinone oxidoreductase cytochrome b small subunit antibody
      • Succinate-ubiquinone reductase membrane anchor subunit antibody
      see all

    Anti-SDHD antibody images

    • Anti-SDHD antibody (ab76607) at 1/500 dilution + immunogen at 0.1 µg

      Secondary
      Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution

      Predicted band size : 17 kDa
      Observed band size : 38 kDa (why is the actual band size different from the predicted?)

    References for Anti-SDHD antibody (ab76607)

    ab76607 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse embryonic stem cells)
    Gel Running Conditions Reduced Denaturing (4-12%)
    Loading amount 20 µg
    Specification Mouse embryonic stem cells
    Blocking step SEA BLOCK Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
    Username

    Svitlana Yablonska

    Verified customer

    Submitted Sep 15 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"