Overview

  • Product nameAnti-SDPR antibody
    See all SDPR primary antibodies
  • Description
    Mouse polyclonal to SDPR
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-425 of Human SDPR (NP_004648)

  • Positive control
    • transfected 293T cell line

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 47 kDa.

Target

  • FunctionMay play a role in targeting PRKCA to caveolae.
  • Tissue specificityHighly expressed in heart and lung, and expressed at lower levels in brain, kidney, liver, pancreas, placenta, and skeletal muscle.
  • Sequence similaritiesBelongs to the PTRF/SDPR family.
  • Post-translational
    modifications
    Phosphorylated on Ser residues.
  • Cellular localizationCytoplasm > cytosol. Membrane > caveola. Colocalizes with CAV1 to caveolae.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cavin 2 antibody
    • Cavin-2 antibody
    • Phosphatidylserine binding protein antibody
    • Phosphatidylserine-binding protein antibody
    • PS p68 antibody
    • PS-p68 antibody
    • Sdpr antibody
    • SDPR_HUMAN antibody
    • SDR antibody
    • Serum deprivation response antibody
    • Serum deprivation response protein antibody
    • Serum deprivation-response protein antibody
    see all

Anti-SDPR antibody images

  • All lanes : Anti-SDPR antibody (ab76867) at 1/500 dilution

    Lane 1 : SDPR transfected 293T cell line
    Lane 2 : non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 47 kDa
    Observed band size : 72 kDa (why is the actual band size different from the predicted?)

References for Anti-SDPR antibody (ab76867)

ab76867 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (12%)
Sample Human Cell lysate - whole cell (Skeletal muscle cells)
Specification Skeletal muscle cells
Treatment N/A
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Oct 21 2014

Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Stem Cells (ES-E14TG2aATCC))
Loading amount 30 µg
Specification Mouse Embryonic Stem Cells (ES-E14TG2aATCC)
Treatment 100 µg/ml fibronectin for 24 hrs
Gel Running Conditions Non-reduced Denaturing (gel 10 %)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
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Submitted Aug 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"