Recombinant
RabMAb

Anti-SDS3 antibody [EPR15000] (ab184555)

Overview

  • Product name
    Anti-SDS3 antibody [EPR15000]
    See all SDS3 primary antibodies
  • Description
    Rabbit monoclonal [EPR15000] to SDS3
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human SDS3 aa 1-150. The exact sequence is proprietary.
    Database link: Q9H7L9

  • Positive control
    • Raji, HeLa, K562 and Molt-4 cell lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab184555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa).
Flow Cyt 1/250. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways (By similarity). May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes.
  • Tissue specificity
    Expressed in various cancer cell ines.
  • Sequence similarities
    Belongs to the SDS3 family.
  • Domain
    The C-terminus is involved in transcriptional repression by HDAC-independent mechanisms.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2400003N08Rik antibody
    • 2410008L21Rik antibody
    • 45 kDa Sin3 associated polypeptide antibody
    • 45 kDa Sin3-associated polypeptide antibody
    • AU067672 antibody
    • FLJ00052 antibody
    • MGC104711 antibody
    • mSds3 antibody
    • RGD1305986 antibody
    • SAP45 antibody
    • SDS3 antibody
    • SDS3, yeast, homolog of antibody
    • SDS3_HUMAN antibody
    • Sin3 histone deacetylase corepressor complex component SDS3 antibody
    • Sin3A associated protein 45kDa antibody
    • Suds3 antibody
    • Suppressor of defective silencing 3 antibody
    • Suppressor of defective silencing 3 homolog (S cerevisiae) antibody
    • Suppressor of defective silencing 3 homolog (SDS3 S cerevisiae) antibody
    • Suppressor of defective silencing 3 protein homolog antibody
    see all

Images

  • All lanes : Anti-SDS3 antibody [EPR15000] (ab184555) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Molt-4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 38 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SDS3  with ab184555 at 1/250 dilution (red) compared to a Rabbit monoclonal isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.

References

ab184555 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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