Overview

  • Product name
  • Description
    Rabbit polyclonal to SEC13L1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Dog, Chimpanzee, Ferret, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 270-320 of Human SEC13L1 (NP_899195.1).

  • Positive control
    • 293T, HeLa, Jurkat, mouse TCMK1 and mouse NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab168824 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 35 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function
    Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles.
  • Sequence similarities
    Belongs to the WD repeat SEC13 family.
    Contains 6 WD repeats.
  • Cellular localization
    Cytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum membrane. Nucleus > nuclear pore complex. In interphase, localizes at both sides of the NPC.
  • Information by UniProt
  • Database links
  • Form
    It is a constituent of the endoplasmic reticulum and the nuclear pore complex. Functions as a component of the nuclear pore complex (NPC) and the COPII coat. At the endoplasmic reticulum, SEC13 is involved in the biogenesis of COPII-coated vesicles. Cellular location: Cytoplasmic vesicle, Endoplasmic reticulum, Membrane, Nuclear pore complex, Nucleus.
  • Alternative names
    • D3S1231E antibody
    • Npp 20 antibody
    • Npp20 antibody
    • Protein SEC13 homolog antibody
    • SEC13 (S. cerevisiae) like 1 antibody
    • Sec13 antibody
    • SEC13 homolog (S. cerevisiae) antibody
    • SEC13 homolog antibody
    • SEC13 like 1 (S. cerevisiae) antibody
    • SEC13 like 1 isoform antibody
    • SEC13 like protein 1 antibody
    • SEC13 protein antibody
    • SEC13 related protein antibody
    • SEC13-like protein 1 antibody
    • SEC13-related protein antibody
    • SEC13_HUMAN antibody
    • SEC13R antibody
    see all

Images

  • All lanes : Anti-SEC13L1 antibody (ab168824) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 35 kDa


    Exposure time : 10 seconds
  • Detection of SEC13L1 in Immunoprecipitates of 293T whole cell lysate (1 mg for IP, 20% of IP loaded) using ab168824 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab168824 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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