Anti-SEC16A antibody (ab111155)

Overview

  • Product nameAnti-SEC16A antibody
    See all SEC16A primary antibodies
  • Description
    Rabbit polyclonal to SEC16A
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from the internal region of Human SEC16A.

  • Positive control
    • Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab111155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • RelevanceSEC16A is required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus. It defines endoplasmic reticulum exit sites (ERES). SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining an ERES. It is required for normal transitional endoplasmic reticulum (tER) organization.
  • Cellular localizationEndoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus membrane; Peripheral membrane protein. Note=SAR1A activity is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation.
  • Database links
  • Alternative names
    • FLJ26737 antibody
    • KIAA0310 antibody
    • OTTHUMP00000022589 antibody
    • OTTHUMP00000022592 antibody
    • OTTHUMP00000022593 antibody
    • p250 antibody
    • Protein SEC16 homolog A antibody
    • Protein transport protein Sec16A antibody
    • RP11 413M3.10 antibody
    • SEC 16 antibody
    • SEC 16A antibody
    • SEC16 antibody
    • SEC16 homolog A (S. cerevisiae) antibody
    • SEC16 homolog A antibody
    • SEC16L antibody
    see all

Anti-SEC16A antibody images

  • ab111155 at 1/50 dilution staining SEC16A in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry. The image on the right is treated with synthesized peptide.

References for Anti-SEC16A antibody (ab111155)

ab111155 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lymphocytes)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Loading amount 10 µg
Specification lymphocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Dec 07 2015


The peptide for ab111155 is now available in the catalog as ab154166.
http://www.abcam.com/index.html?datasheet=154166

Once an order is placed the item will come into stock within 1-2 weeks.

The price is $166.00 for 200 ug.

Thank you for contacting us.

The lab sent me the following information:

The amino acid range of this immunogen is within aa800-1200 of human Protein transport protein Sec16A with accession number O15027 (http://www.uniprot.org/uniprot...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"