Recombinant
RabMAb

Anti-SEC22L1 antibody [EPR12335] (ab181076)

Overview

  • Product name
    Anti-SEC22L1 antibody [EPR12335]
  • Description
    Rabbit monoclonal [EPR12335] to SEC22L1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SEC22L1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O75396

  • Positive control
    • MCF7 and SH-SY5Y cell lysates; Human breast tissue and ovarian carcinoma tissue; SH-SY5Y and MCF7 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab181076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/100 - 1/250.
ICC/IF 1/50 - 1/100.
IP 1/80.
Flow Cyt 1/110.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    SEC22L1 is a member of the SEC22 family of vesicle trafficking proteins. It is a SNARE protein involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
  • Cellular localization
    Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein (By similarity). Golgi apparatus membrane; Single-pass type IV membrane protein (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Database links
  • Alternative names
    • ERS 24 antibody
    • ERS24 antibody
    • SEC22 vesicle trafficking protein homolog B antibody
    • SEC22 vesicle trafficking protein like 1 antibody
    • SEC22B antibody
    • SEC22L 1 antibody
    • Vesicle trafficking protein SEC22b antibody
    see all

Images

  • Anti-SEC22L1 antibody [EPR12335] (ab181076) at 1/2000 dilution + SH-SY5Y cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa

  • Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling SEC22L1 with ab181076 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181076 at 1/80 dilution.
    Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

     

  • Flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling SEC22L1 with ab181076 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SEC22L1 with ab181076 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SEC22L1 with ab181076 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Anti-SEC22L1 antibody [EPR12335] (ab181076) at 1/10000 dilution + MCF7 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 25 kDa

References

ab181076 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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