The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).
1/100 - 1/250.
1/50 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
SEC22L1 is a member of the SEC22 family of vesicle trafficking proteins. It is a SNARE protein involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.
Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type IV membrane protein (By similarity). Golgi apparatus membrane; Single-pass type IV membrane protein (By similarity). Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Immunofluorescent analysis of MCF7 cells (paraformaldehyde-fixed, 4%) labeling SEC22L1 with ab181076 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Western blot analysis of immunoprecipitation pellet from HeLa cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181076 at 1/80 dilution. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling SEC22L1 with ab181076 at 1/110 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling SEC22L1 with ab181076 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling SEC22L1 with ab181076 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Western blot - Anti-SEC22L1 antibody [EPR12335] (ab181076)
Anti-SEC22L1 antibody [EPR12335] (ab181076) at 1/10000 dilution + MCF7 cell lysate at 20 µg
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution