Overview

  • Product name
  • Description
    Rabbit polyclonal to SEC23
  • Tested applications
    Suitable for: IHC-P, ICC, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus laevis, Drosophila melanogaster, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to amino acid residues 1-18 of human Sec23A with C-terminal added cysteine conjugated to KLH. The corresponding sequence is identical in Rat and Mouse Sec23A and differs by one amino acid in Human and Mouse Sec23B.

  • Positive control
    • Human HeLa cells for immunofluorescence and whole cell extracts of Mouse NIH-3T3 and Rat ROS cells for WB.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab50672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 86 kDa).

1 - 2 µg/ml recommended for whole cell extracts of mouse NIH-3T3 and rat ROS cells, applying a chemiluminescent detection reagent.

ICC/IF Use a concentration of 5 - 10 µg/ml.

5 - 10 µg/ml recommended for human HeLa cells.

Target

  • Function
    Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • Involvement in disease
    Defects in SEC23A are the cause of craniolenticulosutural dysplasia (CLSD) [MIM:607812]; also known as cranio-lenticulo-sutural dysplasia. CLSD is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects.
  • Sequence similarities
    Belongs to the SEC23/SEC24 family. SEC23 subfamily.
  • Cellular localization
    Smooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLSD antibody
    • Protein transport protein Sec23A antibody
    • SC23A_HUMAN antibody
    • Sec23 homolog A (S. cerevisiae) antibody
    • SEC23-related protein A antibody
    • sec23a antibody
    see all

Images

  • Human HeLa cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 5 µg/mL Anti-Sec23 antibody. The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugate.


  • Predicted band size : 86 kDa

    Image from Bui M et al, J Biol Chem. 2010 Oct 8;285(41):31590-602. Epub 2010 Jul 29, Fig 1. DOI 10.1074/jbc.M110.101584

References

This product has been referenced in:
  • González C  et al. Axons provide the secretory machinery for trafficking of voltage-gated sodium channels in peripheral nerve. Proc Natl Acad Sci U S A 113:1823-8 (2016). Read more (PubMed: 26839409) »
  • Cho HJ  et al. Leucine-rich repeat kinase 2 regulates Sec16A at ER exit sites to allow ER-Golgi export. EMBO J 33:2314-31 (2014). Read more (PubMed: 25201882) »

See all 3 Publications for this product

Customer reviews and Q&As

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (S2 cells)
Loading amount
30 µg
Specification
S2 cells
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Mar 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse brain)
Specification
mouse brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
PBS, 10%Horse serum, 0,2%BSA, 0,3%Triton as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Aug 22 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (whole brain lysate)
Loading amount
20 µg
Specification
whole brain lysate
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (cultured hippocampal neurons)
Specification
cultured hippocampal neurons
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 30 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
5 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 30 2012

No, 10% is for the separating gel only. Stacking gel is normally 5%.

Thank you for contacting us.
Unfortunately we do not supply HeLa cells samples for ICC/IF or rat ROS cell lysate.
NIH-3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate is sold separately from the antibody and is under the reference ...

Read More

Thank you for your inquiry.
This antibody was used in WB in the following publication:
Bui M et al. Rab32 modulates apoptosis onset and mitochondria-associated membrane (MAM) properties. J Biol Chem 285:31590-602 (2010). WB; Human. (PubMed: 2...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (MEF)
Loading amount
30 µg
Specification
MEF
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Nov 08 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-0.5%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Aug 31 2010

1-10 of 11 Abreviews or Q&A

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