Our Abpromise guarantee covers the use of ab50672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 86 kDa).
1 - 2 µg/ml recommended for whole cell extracts of mouse NIH-3T3 and rat ROS cells, applying a chemiluminescent detection reagent.
|ICC/IF||Use a concentration of 5 - 10 µg/ml.
5 - 10 µg/ml recommended for human HeLa cells.
Image from Bui M et al, J Biol Chem. 2010 Oct 8;285(41):31590-602. Epub 2010 Jul 29, Fig 1. DOI 10.1074/jbc.M110.101584