Synthetic peptide corresponding to amino acid residues 1-18 of human Sec23A with C-terminal added cysteine conjugated to KLH. The corresponding sequence is identical in Rat and Mouse Sec23A and differs by one amino acid in Human and Mouse Sec23B.
Our Abpromise guarantee covers the use of ab50672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 86 kDa).
1 - 2 µg/ml recommended for whole cell extracts of mouse NIH-3T3 and rat ROS cells, applying a chemiluminescent detection reagent.
|ICC/IF||Use a concentration of 5 - 10 µg/ml.
5 - 10 µg/ml recommended for human HeLa cells.
Image from Bui M et al, J Biol Chem. 2010 Oct 8;285(41):31590-602. Epub 2010 Jul 29, Fig 1. DOI 10.1074/jbc.M110.101584