Anti-SEC23 antibody (ab99552)

Overview

  • Product nameAnti-SEC23 antibody
    See all SEC23 primary antibodies
  • Description
    Goat polyclonal to SEC23
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Cow, Dog, Human
  • Immunogen

    Synthetic peptide:

    C-ANRAATTGHVID

    , corresponding to internal sequence amino acids 335-346 of Human SEC23

  • Positive control
    • NIH 3T3 lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab99552 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).

Target

  • FunctionComponent of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • Involvement in diseaseDefects in SEC23A are the cause of craniolenticulosutural dysplasia (CLSD) [MIM:607812]; also known as cranio-lenticulo-sutural dysplasia. CLSD is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects.
  • Sequence similaritiesBelongs to the SEC23/SEC24 family. SEC23 subfamily.
  • Cellular localizationSmooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLSD antibody
    • Protein transport protein Sec23A antibody
    • SC23A_HUMAN antibody
    • Sec23 homolog A (S. cerevisiae) antibody
    • SEC23-related protein A antibody
    • sec23a antibody
    see all

Anti-SEC23 antibody images

  • Anti-SEC23 antibody (ab99552) at 2 µg/ml (incubation time 1 hour) + NIH 3T3 cell lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 86 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

References for Anti-SEC23 antibody (ab99552)

ab99552 has not yet been referenced specifically in any publications.

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Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Panc-1)
Specification Panc-1
Fixative Formaldehyde
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Petrosyan

Verified customer

Submitted Apr 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"