Anti-SEC23IP antibody (ab70338)

Overview

  • Product nameAnti-SEC23IP antibody
    See all SEC23IP primary antibodies
  • Description
    Rabbit polyclonal to SEC23IP
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1 and 50 of human SEC23IP (NP_009121.1)

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notesIP: Use at 1-4µg/mg of lysate.
    WB: 1/2500 - 1/10000. Detects a band of approximately 125 kDa (predicted molecular weight: 111 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPlays a role in the organization of endoplasmic reticulum exit sites.
    • Tissue specificityUbiquitously expressed with stronger levels detected in heart, liver and skeletal muscle.
    • Sequence similaritiesBelongs to the PA-PLA1 family.
      Contains 1 DDHD domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • Cellular localizationCytoplasmic vesicle > COPII-coated vesicle membrane. Endoplasmic reticulum.
    • Information by UniProt
    • Database links
    • Alternative names
      • MSTP053 antibody
      • p125 antibody
      • Phospholipase antibody
      • S23IP_HUMAN antibody
      • Sec23 interacting protein p125 antibody
      • SEC23-interacting protein antibody
      • SEC23IP antibody
      see all

    Anti-SEC23IP antibody images

    • All lanes : Anti-SEC23IP antibody (ab70338) at 0.04 µg/ml

      Lane 1 : HeLa whole cell lysate at 50 µg
      Lane 2 : HeLa whole cell lysate at 15 µg
      Lane 3 : HeLa whole cell lysate at 5 µg
      Lane 4 : 293T whole cell lysate at 50 µg


      Predicted band size : 111 kDa
      Observed band size : 125 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds
    • Detection of Human SEC23IP by Western Blot of Immunprecipitate. Lane 1: HeLa whole cell lysates immunoprecipitated using ab70338 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: control IgG used for immunoprecipitation. All lanes: ab70338 at 1µg/ml. Chemiluminescence with exposure times of 30 seconds

    References for Anti-SEC23IP antibody (ab70338)

    ab70338 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (Liver)
    Loading amount 40 µg
    Specification Liver
    Gel Running Conditions Reduced Non-Denaturing (Native) (4-20%)
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
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    Submitted Feb 15 2013

    Thank you very much for your quick reply.
    I wish you great holidays and a merry Christmas.
    The Abreview is in editing status so that you are able to submit the changes any time.
    I look forward to publish your Abreview with the additional i...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"