Overview

  • Product nameAnti-SEC24A antibody
  • Description
    Rabbit polyclonal to SEC24A
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide between 642~672 amino acids from the central region of human SEC24A.

  • Positive control
    • T47D cytoplasmic lysate Human hepatocarcinoma tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab102660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 120 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/200.

Target

  • RelevanceThe Sec23-Sec24 complex is a component of coat protein II coated vesicles that mediate protein transport from the endoplasmic reticulum in yeast. SEC24A is one of several mammalian proteins that show structural and functional homology to yeast Sec24.
  • Cellular localizationCytoplasm - perinuclear region Endoplasmic reticulum membrane Golgi apparatus membrane
  • Database links
  • Alternative names
    • protein transport protein Sec24A antibody
    • SEC24 family member A S cerevisiae antibody
    • SEC24 related gene family member A antibody
    • SEC24 related protein A antibody
    • SEC24 S cerevisiae related gene family member A antibody
    see all

Anti-SEC24A antibody images

  • ICC/IF image of ab102660 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102660 at 1/200 dilution overnight at +4°C. The secondary antibody (pseudo-colored green) was Alexa Fluor® 488 goat anti- rabbit (ab150081) IgG (H+L) preadsorbed, used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1h at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-SEC24A antibody (ab102660) at 1/100 dilution + T47D cell line lysate at 12.5 µg

    Predicted band size : 120 kDa
  • ab102660, at a 1/50 dilution, staining human SEC24A in hepatocarcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. Followed by peroxidase conjugation of a secondary antibody and DAB staining.

References for Anti-SEC24A antibody (ab102660)

ab102660 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"