Overview

  • Product nameAnti-Sec24C antibody
    See all Sec24C primary antibodies
  • Description
    Rabbit polyclonal to Sec24C
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LTKSPVESTT EPPAVRASEE RLSNGDIYLL ENGLNLFLWV GASVQQGVVQ SLFSVSSFSQ ITSGLSVLPV LDNPLSKKVR, corresponding to C terminal amino acids 954-1033 of Human Sec24C.

  • Positive control
    • Human pancreas tissue; A431 cells; RT 4, U 251 MG, Human liver and tonsil lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Store undiluted.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
WB 1/250 - 1/500.

Target

  • FunctionComponent of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the SEC23/SEC24 family. SEC24 subfamily.
  • Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Protein transport protein Sec24C antibody
    • SC 24C antibody
    • SC24 C antibody
    • SC24C antibody
    • SC24C_HUMAN antibody
    • Sec 24C antibody
    • Sec24 C antibody
    • SEC24 related gene family, member C (S. cerevisiae) antibody
    • SEC24 related protein C antibody
    • SEC24-related protein C antibody
    • SEC24C antibody
    see all

Anti-Sec24C antibody images

  • ab122635 at 4 µg/ml staining SEC24C in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
  • ab122635 at 1/10 dilution staining Sec24C in paraffin-embedded human Pancreas tissue by Immunohistochemistry.
  • All lanes : Anti-Sec24C antibody (ab122635) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique

References for Anti-Sec24C antibody (ab122635)

ab122635 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Monkey Cell (Kidney (COS7 cell line))
Specification Kidney (COS7 cell line)
Permeabilization Yes - saponin
Fixative Paraformaldehyde
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Submitted Dec 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"