Recombinant
RabMAb

Anti-SEC24D [EPR16089] antibody - N-terminal (ab191566)

Overview

  • Product name
    Anti-SEC24D [EPR16089] antibody - N-terminal
    See all SEC24D primary antibodies
  • Description
    Rabbit monoclonal [EPR16089] to SEC24D - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SEC24D aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O94855

  • Positive control
    • SH-SY5Y, HeLa, Raw264.7, PC-12 and NIH/3T3 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab191566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).

Target

  • Function
    Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • Tissue specificity
    Ubiquitously expressed, with higher amounts in placenta, pancreas, heart and liver.
  • Sequence similarities
    Belongs to the SEC23/SEC24 family. SEC24 subfamily.
  • Cellular localization
    Cytoplasm. Cytoplasm > perinuclear region. Endoplasmic reticulum membrane. Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0755 antibody
    • Protein transport protein Sec24D antibody
    • SC24D_HUMAN antibody
    • SEC24 family member D antibody
    • SEC24 related gene family, member D antibody
    • SEC24-related protein D antibody
    • SEC24D antibody
    see all

Images

  • All lanes : Anti-SEC24D [EPR16089] antibody - N-terminal (ab191566) at 1/5000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 113 kDa
    Observed band size: 113 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

  • All lanes : Anti-SEC24D [EPR16089] antibody - N-terminal (ab191566) at 1/1000 dilution

    Lane 1 : Raw264.7 cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 113 kDa
    Observed band size: 113 kDa



    Blocking/Dilution buffer: 5% NFDM /TBST.

References

This product has been referenced in:
  • Keller RB  et al. Monoallelic and biallelic CREB3L1 variant causes mild and severe osteogenesis imperfecta, respectively. Genet Med 20:411-419 (2018). WB . Read more (PubMed: 28817112) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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