The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 133 kDa).
Use at 2-5 µg/mg of lysate.
The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII, in the subcellular structures corresponding to the vesicle transport function.
Cytoplasm. Cytoplasmic vesicle; COPII-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Endoplasmic reticulum membrane; Peripheral membrane protein By similarity. Note: Associates with membranes in a GTP-dependent manner
All lanes : Anti-Sec31A antibody (ab86600) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 133 kDa Observed band size: 133 kDa
Exposure time: 3 seconds
Immunoprecipitation - Sec31A antibody (ab86600)
Detection of Sec31A by Western Blot of Immunprecipitate.
ab86600 at 0.1µg/ml staining Sec31A in HeLa whole cell lysate immunoprecipitated using ab86600 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 seconds.