Anti-SEC61A antibody (ab15037)

Overview

  • Product name
  • Description
    Rabbit polyclonal to SEC61A
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Zebrafish, Rainbow trout
  • Immunogen

    Synthetic peptide:

    KEQSEVGSMGALLF

    conjugated to KLH and BSA, corresponding to amino acids 463-476 of Human SEC61A.

  • Positive control
    • Rat brain microsomal preparation.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 30% Glycerol
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15037 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
WB 1/2500. Predicted molecular weight: 53 kDa.

Target

References

ab15037 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
Loading amount
10 µg
Specification
meiotic egg extract
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 09 2009

Thank you for your enquiry. I have received confirmation that the material used to test the antibody in WB was rat brain microsomes. We are currently in the process of adding the image of the western blot but I can inform you that the blot showed...

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Thank you for your enquiry. I have received confirmation that the material used to test the antibody in WB was rat brain microsomes. We are currently in the process of adding the image of the western blot but I can inform you that the blot showed...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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