Overview

  • Product name
  • Description
    Sheep polyclonal to SEC61A
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 463-476 of Human SEC61A (NP_037468).

  • Positive control
    • A431, HeLa and HepG2 cell lysates for WB. A431 cells for IF/ICC; Cal27 xenograft for IHC-P

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 1X PBS, pH 7
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function
    Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.
  • Sequence similarities
    Belongs to the secY/SEC61-alpha family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • chunp6898 antibody
    • fb62c11 antibody
    • HSEC61 antibody
    • protein transport protein SEC61 alpha subunit antibody
    • protein transport protein Sec61 subunit alpha antibody
    • Protein transport protein Sec61 subunit alpha isoform 1 antibody
    • S61A1_HUMAN antibody
    • SEC 61 antibody
    • SEC 61A antibody
    • Sec61 alpha 1 antibody
    • Sec61 alpha 1 subunit antibody
    • Sec61 alpha 1 subunit (S. cerevisiae) antibody
    • Sec61 alpha-1 antibody
    • SEC61 antibody
    • Sec61 homolog antibody
    • SEC61A1 antibody
    • sec61aa antibody
    see all

Images

  • Anti-SEC61A antibody (ab97537) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 52 kDa
  • Immunofluorescence analysis of methanol-fixed A431 cells, using ab97537 at 1/200 dilution (1) and co-stained with Hoescht 33342 (2).
  • Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using ab97537 at 1/500 dilution.

References

ab97537 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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