Recombinant
RabMAb

Anti-SEC61A antibody [EPR14379] (ab183046)

Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14379
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab183046 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
Flow Cyt 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/40 - 1/60.
IHC-P 1/50 - 1/100.
WB 1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 52 kDa).

Target

  • Function
    Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.
  • Sequence similarities
    Belongs to the secY/SEC61-alpha family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • chunp6898 antibody
    • fb62c11 antibody
    • HSEC61 antibody
    • protein transport protein SEC61 alpha subunit antibody
    • protein transport protein Sec61 subunit alpha antibody
    • Protein transport protein Sec61 subunit alpha isoform 1 antibody
    • S61A1_HUMAN antibody
    • SEC 61 antibody
    • SEC 61A antibody
    • Sec61 alpha 1 antibody
    • Sec61 alpha 1 subunit antibody
    • Sec61 alpha 1 subunit (S. cerevisiae) antibody
    • Sec61 alpha-1 antibody
    • SEC61 antibody
    • Sec61 homolog antibody
    • SEC61A1 antibody
    • sec61aa antibody
    see all

Images

  • Western blot analysis of SEC61A in Daudi cell lysate immunoprecipitated using ab183046 at 1/50 dilution (Lane 1). Lane 2: Negative control.

    Secondary antibody: Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG at 1/1500 dilution.

  • All lanes : Anti-SEC61A antibody [EPR14379] (ab183046) at 1/5000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Daudi cell lysate
    Lane 3 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 52 kDa
    Observed band size : 49 kDa (why is the actual band size different from the predicted?)
  • Flow cytometric analysis of 2% paraformaldehyde-fixed Daudi cells labeling SEC61A with ab183046 at 1/100 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 diution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling SEC61A with ab183046 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling SEC61A with ab183046 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

This product has been referenced in:
  • Levental KR  et al. ?-3 polyunsaturated fatty acids direct differentiation of the membrane phenotype in mesenchymal stem cells to potentiate osteogenesis. Sci Adv 3:eaao1193 (2017). Hamster . Read more (PubMed: 29134198) »
  • Bolar NA  et al. Heterozygous Loss-of-Function SEC61A1 Mutations Cause Autosomal-Dominant Tubulo-Interstitial and Glomerulocystic Kidney Disease with Anemia. Am J Hum Genet 99:174-87 (2016). Read more (PubMed: 27392076) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (brain section)
Permeabilization
Yes - 0.4% Triton
Specification
brain section
Fixative
Formaldehyde
Username

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Verified customer

Submitted Jan 03 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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