The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/40 - 1/60.
1/50 - 1/100.
1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 52 kDa).
Plays a crucial role in the insertion of secretory and membrane polypeptides into the ER. Required for assembly of membrane and secretory proteins. Tightly associated with membrane-bound ribosomes, either directly or through adapter proteins.
Flow cytometric analysis of 2% paraformaldehyde-fixed Daudi cells labeling SEC61A with ab183046 at 1/100 dilution (pink) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 diution.
Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling SEC61A with ab183046 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling SEC61A with ab183046 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Levental KR et al. ?-3 polyunsaturated fatty acids direct differentiation of the membrane phenotype in mesenchymal stem cells to potentiate osteogenesis. Sci Adv3:eaao1193 (2017).
Read more (PubMed: 29134198) »
Bolar NA et al. Heterozygous Loss-of-Function SEC61A1 Mutations Cause Autosomal-Dominant Tubulo-Interstitial and Glomerulocystic Kidney Disease with Anemia. Am J Hum Genet99:174-87 (2016).
Read more (PubMed: 27392076) »