The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 88 kDa (predicted molecular weight: 88 kDa).
RelevanceSEC63 and SEC62 proteins are found to be associated with the ribosome-free SEC61 complex, which is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. Mutations in SEC63 cause autosomal dominant polycystic liver disease.
Cellular localizationEndoplasmic reticulum membrane; Multi pass membrane protein.