All tags secondary-antibodies/alexa-conjugated-secondaries Alexa Fluor® 488-conjugated antibodies

Alexa Fluor® 488-conjugated antibodies

Alexa Fluor® 488 advantages compared to Fluorescein isothiocyanate (FITC) make it the most used green fluorophore for cell staining.

Comparable spectral properties and quantum yield.

Brighter and more photostable conjugates.


FITC

Alexa Fluor® 488

Benefits

Spectrum

Absorption max.: 495 nm

Emission max.:

519 nm

Absorption max.: 495 nm

Emission max.:

519 nm

Comparable spectra means you don’t need to change any settings or filters in your equipment.

Photostability

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Alexa Fluor® 488 high photostability allows more time for observation and image capture.

Brightness

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Detect low-abundance proteins with great sensitivity due to Alexa Fluor® 488 low self-quenching and high fluorescence quantum yield.

A. Annexin V labeled with Alexa Fluor® 488 in frozen rat placenta section by IHC (immunohistochemistry). Green - Anti-Annexin V antibody [EPR3980] (Alexa Fluor® 488) (ab201540); Red - Anti-Tubulin antibody [YOL1/34] (Alexa Fluor® 647) (ab195884); Blue - DAPI.

B. Lamin B1 labeled with Alexa Fluor® 488 in HUVEC cells using Alexa Fluor® 488-conjugated secondary antibody.  Green - Anti-Lamin B1 antibody (ab16048), secondary antibody Goat Anti-Rabbit  IgG H&L (Alexa Fluor® 488) (ab150077); Red - Anti-alpha  Tubulin antibody (ab7291), secondary antibody  Goat anti-mouse  IgG H&L (Alexa Fluor® 647) (ab150115);  Blue - DAPI.

Quick guide to using Alexa Fluor® 488

Excitation laser

Argon ion laser (488 nm)

Emission filter

Confocal microscopy: FITC or Alexa Fluor® 488

Flow cytometry: Band pass (BP) filter 525/30

Compatible fluorophores for multiple staining 

DAPI, Alexa Fluor® 594, and Alexa Fluor® 647

Choosing an Alexa Fluor® 488 secondary antibody

Getting the right secondary antibody to your primary antibody is essential for sensitive protein detection. Make sure you get bright and sensitive staining in immunofluorescence, flow cytometry and immunohistochemistry by choosing one of our Alexa Fluor® 488 conjugated secondary antibodies.

Primary antibody raised in

Use secondary antibody

Rabbit

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150073)

Mouse

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)

Goat

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150129)

Rat

Rabbit Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)

To minimize background due to cross-species reactivity in multiple staining, we recommend the use of Alexa Fluor® pre-adsorbed secondary antibodies. Pre-adsorbed secondary antibodies have gone through an extra purification step to minimize non-specific binding.


Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.