All tags Secondary Antibodies Alexa Fluor® conjugated secondary antibodies

Alexa Fluor® conjugated secondary antibodies

by Javier Conde Vancells

Abreview feedback: 

"It worked perfectly... The intensity of the fluorescence was really great too, strongly recommend."

Anonymous user

Our Alexa Fluor® conjugated secondary antibodies are high quality reagents that are extensively tested to help ensure bright staining and low background. 

Features
Benefits
Brightest dyesThe fluorescence intensity of Alexa Fluor® dyes outperform other spectrally similar dyes
Greater photostabilityIncreased photostability allows for longer periods of image capture
pH insensitivityAlexa Fluor® fluorescence intensity remains high over a broad pH range
Good water solubilityGood water solubility prevents the precipitation and aggregation of Alexa Fluor® conjugated antibodies

Performance:

  • In house manufacturing within our laboratories guarantees consistency and quality.
  • Alexa Fluor® conjugates ensure you make the most of your money by providing lower optimal working dilutions.
  • The wavelength at which Alexa Fluor® conjugates absorb matches the most common operational wavelengths of excitation sources.

Conjugation:

  • We currently offer 9 Alexa Fluor® dyes covering the whole of the spectrum from the UV to the far infrared.
  • The Alexa Fluors® have been conjugated to a large number of secondary antibodies raised in different species including donkey, goat and rabbit.
  • A large number within the range are pre-adsorbed ensuring low species cross reactivity - thus ideal for multi-color analysis.

Validation:

  • Our Alexa Fluor® conjugated secondary antibodies have been tested in a wide range of applications including immunofluorescence/immunocytochemistry (IF/ICC), flow-cytometry, and fluorescent western blot.


Alexa Fluor®Absorption Max (nm)Emission Max (nm)

Extinction
 Coefficient

Quantum Yield
405402421      35,000         -
48849551973,0000.92
555555565155,0000.1
568

578

60388,0000.69
59459061792,0000.66
647650668270,0000.33
680679702184,0000.36
750749775290,0000.12
790784814270,000-


We now have over 200 different Alexa Fluor® secondary antibody conjugates.


Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.