All tags Secondary Antibodies HRP-polymer secondary antibodies

HRP-polymer secondary antibodies

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Use HRP-polymer secondary antibodies to save time, get higher sensitivity and eliminate endogenous biotin background in your immunohistochemistry (IHC) experiments.

HRP-polymer secondary antibodies use micropolymer technology to form smaller detection complexes that allow better tissue penetration and sensitivity. In addition, HRP-polymer secondaries bind more horseradish peroxidase than standard HRP secondary antibodies, increasing signal.



​See why HRP-polymer secondaries are the gold standard for IHC




Biotin

HRP-polymer

Advantages


Protocol

Additional steps for biotin blocking may be required

On average 1 hour less

Simplified protocol means quicker results with HRP-polymer secondaries.

Sensitivity

♦ ♦ ♦

♦ ♦ ♦ ♦

Higher number of HRP molecules bound to HRP-polymer antibodies increase their sensitivity

Specificity

Potential background from endogenous biotin

No background from endogenous biotin 

Using HRP-polymer secondary antibodies eliminates biotin background.

Great for tissues with high biotin.



Example of IHC-parafin using Goat Anti-Mouse IgG H&L secondary antibody (HRP polymer) ab214879

Nuclear detection of Ki67 by immunohistochemistry on breast cancer paraffin embedded tissue using Goat Anti-Mouse IgG H&L secondary antibody (HRP polymer) ab214879

Read next IHC staining protocol for paraffin, frozen and free floating sections


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