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Western blotting (WB) is regarded as one of the most sensitive techniques to detect specific proteins; the final step of a WB experiment is the visualization of specific proteins, with a labeled secondary antibody.
Nineteen high quality alkaline phosphatase (AP) and horseradish peroxidase (HRP) conjugated secondary antibodies optimized for western blot detection against primary antibodies raised in chicken, goat, mouse, rabbit and rat.
All products have been extensively tested and optimized for western blotting.
Cited within the scientific literature.
AP conjugated secondary antibodies can be routinely used at dilutions ranging from 1/5,000 to 1/50,000. Based upon a 1/5,000 dilution in 3ml milk/BSA, you can do over 1500 blots with a single vial of an AP conjugated secondary.
HRP secondary antibodies can be used at dilutions ranging from 1/2,000 to 1/20,000. Assuming a 1/2000 dilution in 3ml milk/BSA, it is possible to perform 600 blots with a single vial of HRP-cojugated secondary.