All tags Secondary Antibodies Secondary antibodies optimized for co-immunoprecipitation western blotting

Secondary antibodies optimized for co-immunoprecipitation western blotting

Conjugated secondary antibodies to resolve primary antibody heavy and light chain detection after co-IP steps.  


Common problems associated with secondary antibodies for western blotting after the co-immumoprecipitation (co-IP) step, are the signals generated as a result of the denatured primary antibody, heavy (~50kDa) or light chains (~35kDa). 

It is therefore difficult to detect your protein of interest should it be found to migrate around either the 50kDa or 35kDa markers.

Figure Legend - a normal secondary HRP antibody to mouse IgG recognizing heavy (H) and light (L) chains of a mouse monoclonal primary (lane 1 - 0.5ug, lane 2 - 0.25ug and lane 3 - 0.125ug

Solution optimized secondary antibodies for co-IP western blotting

To overcome this masking issue, we have developed a range of conjugated secondary antibodies which only recognize primary antibodies in their native state, which eliminates the detection of the denatured primary heavy and/or light chains during Western blotting. 

Furthermore, these optimized secondary antibodies are conjugated to horseradish peroxidase (HRP), alkaline phosphatase (AP) and biotin, all ideal for western blotting.

When choosing a product from the range it is important to take in to consideration the size of your protein of interest - less than 30kDa opt for VeriBlot, or if greater than 30kDa select light chain specific antibodies.

VeriBlot for proteins less than 30kDa

Product nameConjugateProduct code
Anti-goat IgG VeriBlot for IP secondary antibodyHRPab157532
Anti-mouse IgG VeriBlot for IP secondary antibodyBiotinab131367
Anti-mouse IgG VeriBlot for IP secondary antibodyHRPab131368
IgG VeriBlot for IP secondary antibody - bovine, goat, human, mouse, rat, rabbit, sheepAPab131365
IgG VeriBlot for IP secondary antibody - bovine, goat, human, mouse, rat, rabbit, sheepHRPab131366

Light chain specific secondary antibodies for proteins of interest, greater than 30kDa

Product nameConjugateProduct code
Mouse anti-human lambda light chainAPab99810
Rat anti-mouse kappa light chainAPab99616
Rat anti-mouse lambda light chainAPab99624
Rat anti-mouse kappa light chainAPab99631
Mouse anti-rabbit IgG light chainAPab99696
Mouse anti-rat kappa light chainAPab99691
Mouse anti-human lambda light chainHRPab99811
Rat anti-mouse kappa light chainHRPab99617
Rat anti-mouse lambda light chainHRPab99632
Mouse anti-rabbit IgG light chainHRPab99697
Mouse anti-rat kappa light chainHRPab99692

Browse over 2500 high quality secondary antibodies.