All tags secondary-antibodies Secondary antibodies tested with Abcam primary antibodies

Secondary antibodies tested with Abcam primary antibodies

We test our Alexa Fluor® secondary antibodies with recombinant primary antibodies validated in immunofluorescence. To get faster results in immunostaining with your primary antibody, get the tested secondary antibody too.

Alexa Fluor® 488 low self-quenching, high-fluorescence quantum yield dye allows bright and sensitive cell staining. Absorption is at 495 nm and emission maximum is at 519 nm. For fluorescent cell and tissue staining of multiple proteins use in conjugation with secondary antibodies conjugated to Alexa Fluor® 594 or Alexa Fluor® 647 conjugated secondary antibodies.

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

Customer testimonial (5 stars) - 'Excellent! Bright, clear staining observed with secondary antibody ab150077 at a 1:200 dilution.'


Alexa Fluor® 594


Alexa Fluor® 594, absorbs at 561 nm and has an emission maximum at 594 nm, making it ideal for fluorescence cell and tissue staining of multiple proteins with Alexa Fluor® 488 and Alexa Fluor® 647 conjugated secondary antibodies

Goat Anti-Rat IgG H&L (Alexa Fluor® 594) (ab150160)

Customer testimonial (5 stars) - 'Very good antibody. 1st ab used: Rat anti-ApoE'

Alexa Fluor® 647


​Alexa Fluor® 647 absorbs at 650 nm and has an emission maximum at 665 nm spectrum making it ideal for fluorescence cell and tissue staining of multiple proteins with Alexa Fluor® 488 and Alexa Fluor® 594 conjugated secondary antibodies.

Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150075)

Customer testimonial (5 stars) - 'My go-to secondary for IHC. I use a 1:500 dilution in 1 X PBS.'

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Alexa Fluor® is a registered trademark of Life Technologies.  Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.


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