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Products have been extensively tested within our own facilities in applications such as cell imaging, flow cytometry and/or fluorescent western blotting. Robust QC procedures guarantee high performance for each individual product.
The ratio of dye molecules per secondary antibody have been optimized to minimize photobleaching and ensure bright staining. Furthermore, each secondary conjugate undergoes a column separation step to remove free dye thus ensuring minimal background staining.
We now offer Alexa Fluor® conjugated secondary antibodies against specific IgG subclasses (e.g. IgG2a and IgG2b) for the detection of multiple targets to which primary antibodies raised in different species are not available.
A large catalogue of mouse monoclonal primary antibodies has been developed over the years. The possibility to detect subclass-specific mouse monoclonal antibodies in a reliable way adds enormous flexibility to your multiplex experiments.
Image : courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical school.
Embryoid bodies (EBs) obtained upon differentiation of mouse embryonic stem cells.
Primary antibody rabbit anti-laminin. Secondary antibody goat anti-rabbit IgG - H&L (Alexa Fluor® 488) pre-adsorbed (ab150081).
Primary antibody mouse anti-disabled 2. Secondary antibody goat anti-mouse IgG - H&L (Alexa Fluor® 594) pre-adsorbed (ab150120).
Nuclei counter stained with DAPI.
Summary Overlay histogram showing subclass specificity of rat anti-mouse IgG3 gamma chain. Alexa Fluor® 647 (ab172329) reacts specifically with primary mouse IgG3 (red) but not other mouse IgG subtypes namely IgG1 (black), IgG2a (green), IgG2b (pink) and IgM (light blue).
|Color||Primary Ab||Secondary Ab (ab172329)|
ab8989 - mouse monoclonal (isotype IgG1) to Lamin B1
|Green||ab49105 - mouse monoclonal (isotype IgG2a) to alpha 1 catenin||Yes|
|Pink||ab16965 - mouse monoclonal (isotype IgG2b) to thioredoxin||Yes|
|Red||ab60602 - mouse monoclonal (isotype IgG3) to NME2||Yes|
|Light Blue||ab55602 - mouse monoclonal (isotype IgM) to PKM2||Yes|
|Alexa Fluor®||Color in vial*||Absorption Max (nm)||Emission Max (nm)||Emission Color**||Matching Dyes|
|488||Yellow||495||519||Green||Cy2, FITC (fluorescein)|
|555||Pink||555||565||Orange||Cy3, TRITC (Rhodamine)|
|568||Violet||578||603||Orange/ Red||Rhodamine Red|
|647||Blue||650||668||Far Red||APC, Cy5|
* Appearance of the solution in the vial
** Typical emission color seen through a conventional fluorescence microscope with appropriate filters
*** It is not possible to view the light emitted by near-IF fluorescent dyes since human vision is insensitive to light beyond 650 nm approximately.
Alexa Fluor® is a registered trademark of Life Technologies. Alexa Fluor® dye conjugates contain(s) technology licensed to Abcam by Life Technologies.