The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
May play a role in protein trafficking. May act as a linker molecule between the submembranous matrix on the luminal side of zymogen granule membrane (ZGM) and aggregated secretory proteins during granule formation in the TGN.
Highly expressed in liver. Detected at lower levels in colon, ileum and jejunum.
Belongs to the jacalin lectin family.
Secreted > extracellular space > extracellular matrix. Cytoplasmic vesicle lumen. Golgi apparatus lumen. Stored in zymogen granules.