Rabbit polyclonal to Secretory phospholipase A2 Type V
(+) Human, murine, and rat sPLA2 (Type V);
(-) bee venom and human synovial sPLA2 (Type II), iPLA2 (A10 cell lysate), and cPLA2 (HeLa cell lysate);
sPLA2 (Type X) not tested, but is expected to be negative (peptide antigen is 12.5% homologous to murine and rat sPLA2 (Type X) and 25% homologous to human (Type II) and (Type X) sPLA2).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
Heart, placenta and less abundantly, in lung.
Belongs to the phospholipase A2 family.
This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Secretory phospholipase A2 Type V antibody (ab23709)
IHC image of Secretory phospholipase A2 Type V staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23709, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Western blot - Secretory phospholipase A2 Type V antibody (ab23709)
All lanes : Anti-Secretory phospholipase A2 Type V antibody (ab23709) at 1/500 dilution
Lane 1 : Recombinant human sPLA2 (type V) (100 ng) Lane 2 : Recombinant human sPLA2 (type V) (50 ng) Lane 3 : Mouse heart homogenate (50ug)
Predicted band size: 15 kDa Observed band size: 15 kDa
Zhu S et al. High-Density Lipoprotein (HDL) Counter-Regulates Serum Amyloid A (SAA)-Induced sPLA2-IIE and sPLA2-V Expression in Macrophages. PLoS One11:e0167468 (2016).
Read more (PubMed: 27898742) »