Anti-Secretory phospholipase A2 Type V antibody [MCL-3G1] (ab23708)

Overview

  • Product nameAnti-Secretory phospholipase A2 Type V antibody [MCL-3G1]
    See all Secretory phospholipase A2 Type V primary antibodies
  • Description
    Mouse monoclonal [MCL-3G1] to Secretory phospholipase A2 Type V
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • A10 smooth muscle lysate.

Applications

Our Abpromise guarantee covers the use of ab23708 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution.
    WB: Dilute 10µl of reconstituted antibody to 10ml(1/1000) with any buffer suitable for your experiment. Detects a band of approximately 15 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
    • Tissue specificityHeart, placenta and less abundantly, in lung.
    • Sequence similaritiesBelongs to the phospholipase A2 family.
    • Post-translational
      modifications
      This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ca2+ dependent phospholipase A2 antibody
      • Calcium dependent phospholipase A2 antibody
      • Calcium-dependent phospholipase A2 antibody
      • DKFZp686C2294 antibody
      • FRFB antibody
      • Group V phospholipase A2 antibody
      • GV PLA2 antibody
      • gVPLA2 antibody
      • hVPLA(2) antibody
      • MGC46205 antibody
      • OTTHUMP00000044655 antibody
      • PA2G5_HUMAN antibody
      • Phosphatidylcholine 2 acylhydrolase antibody
      • Phosphatidylcholine 2-acylhydrolase 5 antibody
      • Phospholipase A2 group V antibody
      • PLA2 10 antibody
      • PLA2 G5 antibody
      • PLA2-10 antibody
      • Pla2g5 antibody
      • sPLA2 Type V antibody
      see all

    Anti-Secretory phospholipase A2 Type V antibody [MCL-3G1] images

    • All lanes : Anti-Secretory phospholipase A2 Type V antibody [MCL-3G1] (ab23708) at 1/1000 dilution

      Lane 2 : sPLA2 (Type II) at 1 µg
      Lane 3 : A10 smooth muscle lysate (iPLA2) at 20 µg
      Lane 4 : Bee venom sPLA2 at 1 µg
      Lane 5 : Semi-pure cPLA2 at 5 µg
      Lane 6 : sPLA2 (Type V) at 0.04 µg
      Lane 7 : sPLA2 (Type V) at 0.008 µg


      Observed band size : 15 kDa (why is the actual band size different from the predicted?)

    References for Anti-Secretory phospholipase A2 Type V antibody [MCL-3G1] (ab23708)

    ab23708 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"