The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: Dilute 10µl of reconstituted antibody to 10ml(1/1000) with any buffer suitable for your experiment. Detects a band of approximately 15 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle.
Tissue specificityHeart, placenta and less abundantly, in lung.
Sequence similaritiesBelongs to the phospholipase A2 family.
Post-translational modificationsThis enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.
Anti-Secretory phospholipase A2 Type V antibody [MCL-3G1] images
Western blot - Secretory phospholipase A2 Type V antibody [MCL-3G1] (ab23708)
All lanes : Anti-Secretory phospholipase A2 Type V antibody [MCL-3G1] (ab23708) at 1/1000 dilution
Lane 2 : sPLA2 (Type II) at 1 µg Lane 3 : A10 smooth muscle lysate (iPLA2) at 20 µg Lane 4 : Bee venom sPLA2 at 1 µg Lane 5 : Semi-pure cPLA2 at 5 µg Lane 6 : sPLA2 (Type V) at 0.04 µg Lane 7 : sPLA2 (Type V) at 0.008 µg