Overview

  • Product nameAnti-Securin antibody
    See all Securin primary antibodies
  • Description
    Rabbit polyclonal to Securin
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Predicted to work with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Securin.

  • Positive control
    • This antibody gave a positive signal in HEK293 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab26273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
ICC/IF 1/100.

Target

  • FunctionRegulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
  • Tissue specificityExpressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
  • Sequence similaritiesBelongs to the securin family.
  • Developmental stageLow level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded.
  • DomainThe N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
    The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue.
  • Post-translational
    modifications
    Phosphorylated at Ser-165 by CDK1 during mitosis.
    Phosphorylated in vitro by ds-DNA kinase.
    Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • AW555095 antibody
    • C87862 antibody
    • Cut2 antibody
    • EAP 1 antibody
    • EAP1 antibody
    • ESP1 associated protein 1 antibody
    • Esp1-associated protein antibody
    • hPTTG antibody
    • MGC126883 antibody
    • MGC138276 antibody
    • Pds1 antibody
    • Pituitary tumor transforming 1 antibody
    • Pituitary tumor transforming protein 1 antibody
    • Pituitary tumor-transforming 1, isoform CRA_a antibody
    • Pituitary tumor-transforming 1, isoform CRA_b antibody
    • Pituitary tumor-transforming gene 1 antibody
    • Pituitary tumor-transforming gene 1 protein antibody
    • PTTG 1 antibody
    • PTTG antibody
    • PTTG1 antibody
    • PTTG1 protein antibody
    • PTTG1_HUMAN antibody
    • Pttg3 antibody
    • Securin antibody
    • Tumor transforming 1 antibody
    • Tumor transforming protein 1 antibody
    • Tumor-transforming protein 1 antibody
    • TUTR 1 antibody
    • TUTR1 antibody
    see all

Anti-Securin antibody images

  • HeLa cells stained with ab26273 (1/100 dilution). A clear nuclear signal in interphase HeLa cells can be seen.


  • Predicted band size : 22 kDa

    This image is courtesy of an abreview submitted by Andrew Horwitz

    See Abreview

  • Anti-Securin antibody (ab26273) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 22 kDa
    Observed band size : 26 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 minutes
    ab26273 detects a clean 26 kDa band in HEK293 lysate. We would recommend loading 20-40ug of protein when using this antibody. ab26273 has also been tested in mouse lysates and in our hands detects a 70 kDa band which we cannot explain.

References for Anti-Securin antibody (ab26273)

ab26273 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 50 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Feb 05 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - whole cell (sPhi992 strain)
Loading amount 50 µg
Specification sPhi992 strain
Gel Running Conditions Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (MPRO (mouse promyelocytic) cells)
Loading amount 50 µg
Specification MPRO (mouse promyelocytic) cells
Gel Running Conditions Reduced Denaturing (12% Bis-TRIS in MOPS buffer)
Blocking step Odyssey Blocking Agent (Lycor system) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 05 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (lung)
Specification lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 29 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa MCF7 Hs578T)
Loading amount 20 µg
Specification HeLa MCF7 Hs578T
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

Submitted Mar 12 2009

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
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Dr. Kirk McManus

Verified customer

Submitted Oct 03 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"