![Immunocytochemistry/ Immunofluorescence - Securin antibody [DCS-280] (ab3305) image](#Securin-Primary-antibodies-ab3305-6.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Securin antibody [DCS-280] (ab3305) image](#Securin-Primary-antibodies-ab3305-1.jpg)
![Western blot - Securin antibody [DCS-280] (ab3305) image](#Securin-Primary-antibodies-ab3305-5.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Cell Cycle >> Cell Division >> Chromatid Cohesion
Overview
Product name
Anti-Securin antibody [DCS-280]
See all Securin products (9) ...
Description
Mouse monoclonal [DCS-280] to Securin
Tested applications
ICC/IF, WB, IHC-P, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Human recombinant full length Pds 1 protein.
Positive control
HeLa cells. SVEC4-10 cells. Tonsil tissue sections.
General notes
LocusID: 9232
OMIM: 604147
SwissProt accession no.: O95997
Alternative names: Pituitary Tumor Transforming Gene 1; PTTG1; PTTG; Esp1-associated protein 1; EAP 1; Tumor-Transforming 1; TUTR 1; Securin; Pds 1; Pds 1.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
DCS-280
Isotype
IgG2a
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3305 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/50 - 1/200.
WB: Use at an assay dependent dilution.
IHC-P: Use a concentration of 1 µg/ml
IP: Use at an assay dependent dilution. (See Abreview.)
Target
Function
Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation.
Tissue specificity
Expressed at low level in most tissues, except in adult testis, where it is highly expressed. Overexpressed in many patients suffering from pituitary adenomas, primary epithelial neoplasias, and esophageal cancer.
Sequence similarities
Belongs to the securin family.
Developmental stage
Low level during G1 and S phases. Peaks at M phase. During anaphase, it is degraded.
Domain
The N-terminal destruction box (D-box) acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.
The TEK-boxes are required for 'Lys-11'-linked ubiquitination and facilitate the transfer of the first ubiquitin and ubiquitin chain nucleation. TEK-boxes may direct a catalytically competent orientation of the UBE2C/UBCH10-ubiquitin thiolester with the acceptor lysine residue.
Post-translational
modifications
Phosphorylated at Ser-165 by CDK1 during mitosis.
Phosphorylated in vitro by ds-DNA kinase.
Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionO95997
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AW555095 antibody
- C87862 antibody
- Cut2 antibody
see all
Database links
- Entrez Gene: 9232 Human
- Entrez Gene: 30939 Mouse
- Entrez Gene: 64193 Rat
- Omim: 604147 Human
- SwissProt: O95997 Human
- SwissProt: Q6IAL9 Human
- SwissProt: Q3Y5K4 Mouse
- SwissProt: Q5SRU0 Mouse
see all
Anti-Securin antibody [DCS-280] images:
Immunocytochemistry/ Immunofluorescence - Securin antibody [DCS-280] (ab3305)
![Immunocytochemistry/ Immunofluorescence - Securin antibody [DCS-280] (ab3305)](/ps/datasheet/images/3/ab3305/Securin-Primary-antibodies-ab3305-6.jpg)
ab3305 (1/200) staining Securin in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde (4%, 10 minutes), permeabilised (0.5% Triton X100 in PBS) and counterstained with DAPI in order to highlight the nucleus (red). Please refer to Abreview for further experimental details.
This image is courtesy of an Abreview submitted by Dr Kirk McManus
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Securin antibody [DCS-280] (ab3305)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Securin antibody [DCS-280] (ab3305)](/ps/datasheet/images/3/ab3305/Securin-Primary-antibodies-ab3305-1.jpg)
ab3305 (1µg/ml) staining Securin in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic and nuclear staining of cells of the germinal centre.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Western blot - Securin antibody [DCS-280] (ab3305)
![Western blot - Securin antibody [DCS-280] (ab3305)](/ps/datasheet/images/3/ab3305/Securin-Primary-antibodies-ab3305-5.jpg)
Anti-Securin antibody [DCS-280] (ab3305) at 2 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
Western blot - Anti-Securin antibody [DCS-280] (ab3305)
![Western blot - Anti-Securin antibody [DCS-280] (ab3305)](/ps/datasheet/images/3/ab3305/Securin-Primary-antibodies-ab3305-20.jpg)
All lanes : Anti-Securin antibody [DCS-280] (ab3305) at 1/1000 dilution
Lane 1 : AtT20 (mouse pituitary) cell lysate
Lane 2 : AtT20 (mouse pituitary) cell lysate
Lane 3 : AtT20 (mouse pituitary) cell lysate
Lane 4 : AtT20 (mouse pituitary) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
HRP-conjugated Sheep anti-Mouse polyclonal at 1/1000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 27 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 seconds
Cells were transfected with different genes.
This image is courtesy of an Anonymous Abreview.
References for Anti-Securin antibody [DCS-280] (ab3305)
This product has been referenced in:
- Holt JEet al. The APC/C activator FZR1 coordinates the timing of meiotic resumption during prophase I arrest in mammalian oocytes. Development 138:905-13 (2011).Read more (PubMed: 21270054) »
- Chang HYet al. Essential role of protein phosphatase 2A in metaphase II arrest and activation of mouse eggs shown by okadaic acid, dominant negative protein phosphatase 2A, and FTY720. J Biol Chem 286:14705-12 (2011).Read more (PubMed: 21383018) »
See all 27 publications for this product
Publishing research using ab3305? Please let us know so that we can cite the reference in this datasheet
![Securin antibody [DCS-280] for ICC/IF in Human (3305)](/ps/Reviews/Images/ab9231_45153.jpg)
![Securin antibody [DCS-280] for IP in Human (3305)](/ps/Reviews/Images/ab12283_67163.jpg)
![Anti-Securin antibody [DCS-280] for Western blot in Mouse (3305)](/ps/datasheet/images/3/ab3305/Securin-Primary-antibodies-ab3305-12.JPG)
![Anti-Securin antibody [DCS-280] for Western blot in Rat (3305)](/ps/datasheet/images/3/ab3305/Securin-Primary-antibodies-ab3305-18.png)
![Securin antibody [DCS-280] for ICC/IF in Human (3305)](/ps/Reviews/Images/ab9933_51954.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"