Anti-Selenium Binding Protein 1 antibody (ab72249)

Overview

  • Product name
    Anti-Selenium Binding Protein 1 antibody
    See all Selenium Binding Protein 1 primary antibodies
  • Description
    Rabbit polyclonal to Selenium Binding Protein 1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Xenopus laevis, Non human primates
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Selenium Binding Protein 1.

    (Peptide available as ab90221.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Liver; Colon; Spleen.

Properties

Applications

Our Abpromise guarantee covers the use of ab72249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport.
  • Tissue specificity
    Highly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated in brain and blood from schizophrenia patients.
  • Sequence similarities
    Belongs to the selenium-binding protein family.
  • Post-translational
    modifications
    Phosphorylated.
    The N-terminus is blocked.
  • Cellular localization
    Nucleus. Cytoplasm > cytosol. Membrane. May associate with Golgi membrane. May associate with the membrane of autophagosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 56 kDa selenium binding protein antibody
    • 56 kDa selenium-binding protein antibody
    • hSBP antibody
    • LPSB antibody
    • SBP antibody
    • SBP1 antibody
    • SBP1_HUMAN antibody
    • SBP56 antibody
    • Selenbp1 antibody
    • Selenbp2 antibody
    • Selenium binding protein 2 antibody
    • Selenium binding protein1 antibody
    • Selenium-binding protein 1 antibody
    • SELNBP1 antibody
    • SP56 antibody
    see all

Images

  • All lanes : Anti-Selenium Binding Protein 1 antibody (ab72249) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ICC/IF image of ab72249 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72249, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa and MCF7 cells at 5µg/ml.

References

ab72249 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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