Anti-Selenium Binding Protein 1 antibody (ab90135)

Overview

  • Product nameAnti-Selenium Binding Protein 1 antibody
    See all Selenium Binding Protein 1 primary antibodies
  • Description
    Rabbit polyclonal to Selenium Binding Protein 1
  • Tested applicationsSuitable for: WB, ICC/IF, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Selenium Binding Protein 1.

    (Peptide available as ab90251.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Liver: Human Colon; Human Spleen; Mouse Liver; Mouse Lung; Rat Liver.

Properties

Applications

Our Abpromise guarantee covers the use of ab90135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionSelenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport.
  • Tissue specificityHighly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated in brain and blood from schizophrenia patients.
  • Sequence similaritiesBelongs to the selenium-binding protein family.
  • Post-translational
    modifications
    Phosphorylated.
    The N-terminus is blocked.
  • Cellular localizationNucleus. Cytoplasm > cytosol. Membrane. May associate with Golgi membrane. May associate with the membrane of autophagosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 56 kDa selenium binding protein antibody
    • 56 kDa selenium-binding protein antibody
    • hSBP antibody
    • LPSB antibody
    • SBP antibody
    • SBP1 antibody
    • SBP1_HUMAN antibody
    • SBP56 antibody
    • Selenbp1 antibody
    • Selenbp2 antibody
    • Selenium binding protein 2 antibody
    • Selenium binding protein1 antibody
    • Selenium-binding protein 1 antibody
    • SELNBP1 antibody
    • SP56 antibody
    see all

Anti-Selenium Binding Protein 1 antibody images

  • All lanes : Anti-Selenium Binding Protein 1 antibody (ab90135) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human colon tissue lysate - total protein (ab30051)
    Lane 3 : Human spleen tissue lysate - total protein (ab29699)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab90135 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab90135, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, and Hek293 cells at 5µg/ml.
  • IHC image of ab90135 staining in Normal Human Liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90135, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-Selenium Binding Protein 1 antibody (ab90135) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Lung (Mouse) Tissue Lysate
    Lane 3 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa


    Exposure time : 1 minute
  • Selenium Binding Protein 1 was immunoprecipitated using 0.5mg Mouse Liver tissue lysate, 5µg of Rabbit polyclonal to Selenium Binding Protein 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Liver tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab90135.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 52kDa; Selenium Binding Protein 1

References for Anti-Selenium Binding Protein 1 antibody (ab90135)

This product has been referenced in:
  • Chen F  et al. Selenium-binding protein 1 in head and neck cancer is low-expression and associates with the prognosis of nasopharyngeal carcinoma. Medicine (Baltimore) 95:e4592 (2016). IHC-P ; Human . Read more (PubMed: 27583873) »
  • Song H  et al. Distribution and inhibition effect of Seleno-L-Methionine on 4T1 mouse mammary carcinoma. Int J Physiol Pathophysiol Pharmacol 7:76-86 (2015). Mouse . Read more (PubMed: 26330897) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Stomach)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9.0
Permeabilization Yes - Wash Buffer with Tween
Specification Stomach
Fixative Paraformaldehyde
Username

Mr. Rudolf Jung

Verified customer

Submitted Nov 02 2015

Application Western blot
Loading amount 12 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Human Cell lysate - other (Epicardial adipose tissue conditioned media)
Specification Epicardial adipose tissue conditioned media
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jan 21 2014

Thank you for contacting us. I had a look at the AP56 protein in mouse which is the selenium liver binding protein in mouse, this appears to be a different target. I did not see an alternative name for AP56 on the Swiss Prot page, but the molecular wei...

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