Anti-Selenophosphate synthetase 2 antibody (ab153878)

Overview

  • Product name
    Anti-Selenophosphate synthetase 2 antibody
    See all Selenophosphate synthetase 2 primary antibodies
  • Description
    Rabbit polyclonal to Selenophosphate synthetase 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Dog, Pig, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 116-429 of Human Selenophosphate synthetase 2 (UniProt: Q99611).

  • Positive control
    • A431, H1299, HeLa and HepG2 whole cell lysates; A431 cells; Human OVCA tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab153878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 47 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co translationally incorporated into selenoproteins at in frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop.
  • Database links
  • Alternative names
    • Selenide, water dikinase 2 antibody
    • Selenium donor protein 2 antibody
    • selenophosphate synthetase 2 antibody
    • SEPHS2 antibody
    • SPS2 antibody
    • SPS2b antibody
    see all

Images

  • Anti-Selenophosphate synthetase 2 antibody (ab153878) at 1/1000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size: 47 kDa



    10% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells, labeling Selenophosphate synthetase 2 with ab153878 at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded Human OVCA tissue, labeling Selenophosphate synthetase 2 with ab153878 at 1/100 dilution

References

ab153878 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole body)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
whole body
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Feb 21 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (ovarian cancer)
Permeabilization
Yes - Ethanol 70%
Specification
ovarian cancer
Fixation
Ethanol 70%
Username

Osnat Ashur-Fabian

Verified customer

Submitted Nov 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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