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Rabbit polyclonal to Selenophosphate synthetase 2
Predicted to work with:
Cow, Dog, Pig, Monkey
Recombinant fragment, corresponding to a region within amino acids 116-429 of Human Selenophosphate synthetase 2 (UniProt: Q99611).
A431, H1299, HeLa and HepG2 whole cell lysates; A431 cells; Human OVCA tissue.
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 47 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/100 - 1/1000.
This protein encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co translationally incorporated into selenoproteins at in frame UGA codons. This protein itself contains a selenocysteine residue in its predicted active site. The 3' UTR of the gene has a stem loop secondary structure called a selenocysteine insertion sequence (SECIS) element, which allows UGA to direct the incorporation of selenocysteine rather than signal a translational stop.
Selenide, water dikinase 2 antibody
Selenium donor protein 2 antibody
selenophosphate synthetase 2 antibody
Western blot - Anti-Selenophosphate synthetase 2 antibody (ab153878)
Anti-Selenophosphate synthetase 2 antibody (ab153878) at 1/1000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 47 kDa 10% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-Selenophosphate synthetase 2 antibody (ab153878)
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells, labeling Selenophosphate synthetase 2 with ab153878 at 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Selenophosphate synthetase 2 antibody (ab153878)
Immunohistochemical analysis of paraffin-embedded Human OVCA tissue, labeling Selenophosphate synthetase 2 with ab153878 at 1/100 dilution
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"