Overview

  • Product name
    Anti-SELH antibody
  • Description
    Rabbit polyclonal to SELH
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster
  • Immunogen

    Recombinant fragment, corresponding to amino acids 68-122 of Human SELH (Q8IZQ5).

  • Positive control
    • Human bronchus tissue
  • General notes
    The antibody solution should be gently mixed before use.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG

Applications

Our Abpromise guarantee covers the use of ab151023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Target

Images

  • Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling SELH with ab151023 shows positive staining in the nucleus but excluded from the nucleoli.
  • Immunohistochemical analysis of paraffin embedded Human bronchus tissue labeling SELH with ab151023 antibody at 1/200.

References

This product has been referenced in:
  • Hiller F  et al. Differential acute effects of selenomethionine and sodium selenite on the severity of colitis. Nutrients 7:2687-706 (2015). Read more (PubMed: 25867950) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (whole body)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
40 µg
Specification
whole body
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

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Verified customer

Submitted Feb 21 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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