Overview

  • Product name
  • Description
    Rabbit polyclonal to SELK
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: KRRSYGNSSD SRYDDGRGPP GNPPRRMGRI NHLRGPS, corresponding to amino acids 48-84 of Human SELK.

  • Positive control
    • RT 4, U 251 MG, Human liver and Human tonsil lysates; Human salivary gland tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab121276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121276 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-SELK antibody (ab121276) at 1/250 dilution

    Lane 1 : RT 4 lysate
    Lane 2 : U 251 MG lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver lysate
    Lane 5 : Human tonsil lysate

    Developed using the ECL technique
  • ab121276 at 1/40 dilution staining SELK in Paraffin-embedded Human Salivary gland tissue by Immunohistochemistry.

References

ab121276 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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