Anti-SEMA4A antibody (ab70178)
Key features and details
- Rabbit polyclonal to SEMA4A
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-SEMA4A antibody
See all SEMA4A primary antibodies -
Description
Rabbit polyclonal to SEMA4A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat -
Immunogen
A synthesized peptide derived from the internal region of human SEMA4A.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70178 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Predicted molecular weight: 84 kDa.
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IHC-P |
1/50 - 1/100.
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Notes |
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WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. -
Involvement in disease
Defects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) [MIM:610282]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
Defects in SEMA4A are the cause of cone-rod dystrophy type 10 (CORD10) [MIM:610283]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 64218 Human
- Entrez Gene: 20351 Mouse
- Entrez Gene: 310630 Rat
- Omim: 607292 Human
- SwissProt: Q9H3S1 Human
- SwissProt: Q62178 Mouse
- Unigene: 408846 Human
- Unigene: 439752 Mouse
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Alternative names
- CORD10 antibody
- RP11 54H19 2 antibody
- RP35 antibody
see all
Images
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All lanes : Anti-SEMA4A antibody (ab70178) at 1/500 dilution
Lane 1 : Extracts from COS-7 cells
Lane 2 : Extracts from Jurkat cells
Lane 3 : Extracts from COS-7 cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 84 kDa
Observed band size: 84 kDa
Additional bands at: 117 kDa, 26 kDa. We are unsure as to the identity of these extra bands.
One other band detected greater then 117 kDa. -
Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab70178 at 1/50-1/100 dilution.
Left image un-treated.Right image treated with immunizing peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab70178 has been referenced in 3 publications.
- Sukumaran SK et al. Whole transcriptome profiling of taste bud cells. Sci Rep 7:7595 (2017). PubMed: 28790351
- Wang L et al. Expression of Semaphorin 4A and its potential role in rheumatoid arthritis. Arthritis Res Ther 17:227 (2015). ELISA ; Human . PubMed: 26303122
- Vadasz Z et al. The involvement of immune semaphorins and neuropilin-1 in lupus nephritis. Lupus 20:1466-73 (2011). IHC-P ; Human . PubMed: 21951945