Overview

  • Product nameAnti-SEMA4A antibody
    See all SEMA4A primary antibodies
  • Description
    Rabbit polyclonal to SEMA4A
  • SpecificityThis antibody detects endogenous levels of total SEMA4A protein.
  • Tested applicationsSuitable for: IP, WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from the internal region of human SEMA4A.

  • Positive control
    • Extracts from COS-7 and Jurkat cells. Human brain tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab70178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/500 - 1/1000. Predicted molecular weight: 84 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionInhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • Involvement in diseaseDefects in SEMA4A are the cause of retinitis pigmentosa type 35 (RP35) [MIM:610282]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in SEMA4A are the cause of cone-rod dystrophy type 10 (CORD10) [MIM:610283]. CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa.
  • Sequence similaritiesBelongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CORD10 antibody
    • RP11 54H19 2 antibody
    • RP35 antibody
    • SEM4A_HUMAN antibody
    • Sema B antibody
    • Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain 4A antibody
    • Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4A antibody
    • Sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) antibody
    • Sema4a antibody
    • SEMAB antibody
    • Semaphorin 4A precursor antibody
    • Semaphorin B antibody
    • Semaphorin-4A antibody
    • Semaphorin-B antibody
    • SEMB antibody
    see all

Anti-SEMA4A antibody images

  • All lanes : Anti-SEMA4A antibody (ab70178) at 1/500 dilution

    Lane 1 : Extracts from COS-7 cells
    Lane 2 : Extracts from Jurkat cells
    Lane 3 : Extracts from COS-7 cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 84 kDa
    Observed band size : 84 kDa
    Additional bands at : 117 kDa,26 kDa. We are unsure as to the identity of these extra bands.
  • SEMA4A was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to SEMA4A and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab70178.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: Band: 90kDa: SEMA4A.
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab70178 at 1/50-1/100 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.
  • ICC/IF image of ab70178 stained SKNSH cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70178, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-SEMA4A antibody (ab70178)

This product has been referenced in:
  • Wang L  et al. Expression of Semaphorin 4A and its potential role in rheumatoid arthritis. Arthritis Res Ther 17:227 (2015). ELISA ; Human . Read more (PubMed: 26303122) »
  • Vadasz Z  et al. The involvement of immune semaphorins and neuropilin-1 in lupus nephritis. Lupus 20:1466-73 (2011). IHC-P ; Human . Read more (PubMed: 21951945) »

See all 2 Publications for this product

Product Wall

Thank you for your email. I have more information regarding the immunogen: The antibody binds to the PSI domain. Alignment of our immunogen sequence with human SEMA4D ACC# http://www.uniprot.org/uniprot/Q92854 shows a 25% identity in an 8 residue...

Read More

Thank you for your reply.

Kate is currently away from the office but I will try to help in your case as best I can.

Having reviewed the case I think you may be detecting the expected protein. In the very first images you shared with u...

Read More

Thank you for taking the time to contact us with these details. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for yo...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"