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Rabbit polyclonal to SEMA4B
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to a region within N terminal amino acids 182-231 (KGRCPFDPNF KSTALVVDGE LYTGTVSSFQ GNDPAISRSQ SLRPTKTESS) of human SEMA4B (NP_064595).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
SEMA4B belongs to the semaphorin family and contains 1 Ig-like C2-type (immunoglobulin-like) domain, 1 PSI domain and 1 Sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B antibody
Western blot - SEMA4B antibody (ab81130)
Western blot - Anti-SEMA4B antibody (ab81130)
Anti-SEMA4B antibody (ab81130) at 1.6 µg/ml + MIA-PACA2 cells
Predicted band size:
WB Suggested Anti-WBP11 antibody (
ab85563) Titration: 2 µg/ml Positive Control: Human NT-2 cells and Mouse WT brain.
Secondary antibody -
anti-rabbit HRP ( ab6721)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"