Overview

  • Product name
  • Description
    Rabbit polyclonal to SEMA4B
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 182-231 (KGRCPFDPNF KSTALVVDGE LYTGTVSSFQ GNDPAISRSQ SLRPTKTESS) of human SEMA4B (NP_064595).

  • Positive control
    • Fetal membrane lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified by peptide affinity chromatography method.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Relevance
    SEMA4B belongs to the semaphorin family and contains 1 Ig-like C2-type (immunoglobulin-like) domain, 1 PSI domain and 1 Sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • Cellular localization
    Membrane
  • Database links
  • Alternative names
    • KIAA1745 antibody
    • MGC131831 antibody
    • Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B antibody
    • Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain 4B antibody
    • SEMA4B antibody
    • SEMAC antibody
    • Semaphorin 4B antibody
    • SemC antibody
    see all

Images

  • Anti-SEMA4B antibody (ab81130) at 1 µg/ml + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Anti-SEMA4B antibody (ab81130) at 1.6 µg/ml + MIA-PACA2 cells

    Predicted band size : 93 kDa

    WB Suggested Anti-WBP11 antibody (ab85563) Titration: 2 µg/ml Positive Control: Human NT-2 cells and Mouse WT brain.

    Secondary antibody - anti-rabbit HRP (ab6721)

References

ab81130 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
No
Sample
Mouse Cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Gating Strategy
live cells in fsc vs ssc
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.2% BSA, 0.1% NaAz in PBS
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Verified customer

Submitted Apr 30 2014

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification
mouse embryonic stem cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Verified customer

Submitted Jan 07 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HEK293 cells transfected with mouse Sema4B express)
Specification
HEK293 cells transfected with mouse Sema4B express
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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