Overview

  • Product nameAnti-SEMA4B antibody
    See all SEMA4B primary antibodies
  • Description
    Rabbit polyclonal to SEMA4B
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 182-231 (KGRCPFDPNF KSTALVVDGE LYTGTVSSFQ GNDPAISRSQ SLRPTKTESS) of human SEMA4B (NP_064595).

  • Positive control
    • Fetal membrane lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified by peptide affinity chromatography method.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab81130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 93 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • RelevanceSEMA4B belongs to the semaphorin family and contains 1 Ig-like C2-type (immunoglobulin-like) domain, 1 PSI domain and 1 Sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • Cellular localizationMembrane
  • Database links
  • Alternative names
    • KIAA1745 antibody
    • MGC131831 antibody
    • Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B antibody
    • Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain 4B antibody
    • SEMA4B antibody
    • SEMAC antibody
    • Semaphorin 4B antibody
    • SemC antibody
    see all

Anti-SEMA4B antibody images

  • Anti-SEMA4B antibody (ab81130) at 1 µg/ml + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 93 kDa
    Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Secondary antibody - anti-rabbit HRP (ab6721)

  • Anti-SEMA4B antibody (ab81130) at 1.6 µg/ml + MIA-PACA2 cells

    Predicted band size : 93 kDa

    WB Suggested Anti-WBP11 antibody (ab85563) Titration: 2 µg/ml Positive Control: Human NT-2 cells and Mouse WT brain.

    Secondary antibody - anti-rabbit HRP (ab6721)

References for Anti-SEMA4B antibody (ab81130)

ab81130 has not yet been referenced specifically in any publications.

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization No
Sample Mouse Cell (mouse embryonic stem cells)
Specification mouse embryonic stem cells
Gating Strategy live cells in fsc vs ssc
Preparation Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.2% BSA, 0.1% NaAz in PBS
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Submitted Apr 30 2014

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cells)
Specification mouse embryonic stem cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
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Submitted Jan 07 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (HEK293 cells transfected with mouse Sema4B express)
Specification HEK293 cells transfected with mouse Sema4B express
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted Dec 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"