Overview

  • Product name
  • Description
    Rabbit polyclonal to SEMA4F
  • Specificity
    ab86549 recognizes isoform 2.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 36-85 (VPRSVPRTSL PISEADSCLT RFAVPHTYNY SVLLVDPASH TLYVGARDTI) of human SEMA4F (AAH18361; O95754-2).

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86549 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 66 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

Images

  • Anti-SEMA4F antibody (ab86549) at 1 µg/ml (in 5% skim milk / PBS buffer) + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 66 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)

References

ab86549 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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