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Products:Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
Overview
Product name
Anti-Sema6A antibody
See all Sema6A products (3) ...
Description
Rabbit polyclonal to Sema6A
Tested applications
Cross reactivity
Predicted to work with
Mouse, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Sema6A.
(Peptide available as ab27882.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Neuroscience >> Neurology process >> Growth and Development >> Axonal Guidance Proteins
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Western blot - Sema6A antibody (ab34818)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 1 µg/ml.
ELISA: This antibody gave a positive result in ELISA against the immunizing peptide (ab27882).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
SEMA6A belongs to a subfamily characterized by an extracellular semaphorin domain, a transmembrane domain, and a long cytoplasmic tail. Members of this class can repel sympathetic and dorsal root ganglion axons in vitro, consistent with a traditional role as guidance signals. However, the length of the cytoplasmic tail, which includes an EVL-binding site in SEMA6A and an Src-binding site in SEMA6B, suggests that these semaphorins may also function as receptors. SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection. SEMA6A directly links the Ena/VASP and the semaphorin protein families since the SEMA6A protein is capable of selective binding to the protein EVL (Ena/VASP-like protein).
Cellular localization
Type I membrane protein
Alternative names
- Sema Q antibody
- Sema VIA antibody
- SEMA6A-1 antibody
see all
Database links
- Entrez Gene: 57556 Human
- Entrez Gene: 20358 Mouse
- Omim: 605885 Human
- SwissProt: Q9H2E6 Human
- SwissProt: O35464 Mouse
- SwissProt: Q8BUT0 Mouse
- SwissProt: Q8BXZ7 Mouse
- Unigene: 156967 Human
see all
Anti-Sema6A antibody images:
Western blot - Sema6A antibody (ab34818)
Anti-Sema6A antibody (ab34818) at 1 µg/ml + SEMA6A Recombinant protein (Abnova) at 0.1 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
This antibody detects a Sema6A recombinant protein in Western blot.
References for Anti-Sema6A antibody (ab34818)
ab34818 has not yet been referenced specifically in any publications.
Publishing research using ab34818? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"