The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 130 kDa (predicted molecular weight: 100 kDa).
RelevanceSEMA6C belongs to a subfamily characterized by an extracellular semaphorin domain, a transmembrane domain, and a long cytoplasmic tail. Members of this class can repel sympathetic and dorsal root ganglion axons in vitro, consistent with a traditional role as guidance signals. SEMA6C shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro and may be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. SEMA6C may also be involved in the maintenance and remodeling of neuronal connections.
Cellular localizationMembrane; Single-pass type I membrane protein.