Anti-Semaphorin 3A antibody (ab23393)
Key features and details
- Rabbit polyclonal to Semaphorin 3A
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
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Overview
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Product name
Anti-Semaphorin 3A antibody
See all Semaphorin 3A primary antibodies -
Description
Rabbit polyclonal to Semaphorin 3A -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
Use at an assay dependent concentration.
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WB | (3) |
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa). |
Target
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Function
Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1. -
Involvement in disease
Hypogonadotropic hypogonadism 16 with or without anosmia -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Domain
Strong binding to neuropilin is mediated by the carboxy third of the protein. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 10371 Human
- Entrez Gene: 29751 Rat
- Omim: 603961 Human
- SwissProt: Q14563 Human
- SwissProt: Q63548 Rat
- Unigene: 252451 Human
- Unigene: 44410 Rat
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Alternative names
- Coll 1 antibody
- Coll1 antibody
- Collapsin 1 antibody
see all
Images
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Ab23393 staining human normal ileum. Staining is localized to the cytoplasm.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
Lanes 1-2 : Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution
Lanes 3-4 : Anti-Semaphorin 3A antibody (ab25999)
Lane 1 : Neonatal rat brain
Lanes 2 & 4 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
Lane 3 : Neonatal rat brain
Predicted band size: 89 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (58)
ab23393 has been referenced in 58 publications.
- Su Y et al. Astrocyte endfoot formation controls the termination of oligodendrocyte precursor cell perivascular migration during development. Neuron 111:190-201.e8 (2023). PubMed: 36384142
- Zheng Y et al. Regulation of Semaphorin3A in the process of cutaneous wound healing. Cell Death Differ 29:1941-1954 (2022). PubMed: 35347234
- Jin T et al. Developmental Hypothyroidism Influences the Development of the Entorhinal-Dentate Gyrus Pathway of Rat Offspring. Endocrinol Metab (Seoul) 37:290-302 (2022). PubMed: 35390249
- Wang T et al. Delivery of miR-654-5p via SonoVue Microbubble Ultrasound Inhibits Proliferation, Migration, and Invasion of Vascular Smooth Muscle Cells and Arterial Thrombosis and Stenosis through Targeting TCF21. Oxid Med Cell Longev 2022:4757081 (2022). PubMed: 35910838
- Obata H et al. Age‑related differences for expression of the nerve‑specific proteins after peripheral nerve injury. Exp Ther Med 24:682 (2022). PubMed: 36185767