Overview

  • Product nameAnti-Semaphorin 3A antibody
    See all Semaphorin 3A primary antibodies
  • Description
    Rabbit polyclonal to Semaphorin 3A
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IF, IHC-FoFr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human, Xenopus laevis, Zebrafish, Japanese ricefish, Apteronotus leptorhynchus
  • Immunogen

    Synthetic peptide (coupled to carrier protein):

    RDGPNYQWVPYQGR

    , corresponding to amino acids 359-372 of Human Semaphorin 3A (Peptide available as ab42768).

  • Positive control
    • Rat brain lysate for SDS PAGE immunoblots.

Properties

Applications

Our Abpromise guarantee covers the use of ab23393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 19443185
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 18574596
WB 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 89 kDa).Can be blocked with Active human Semaphorin 3A peptide (ab88818).
ELISA 1/2000.

Target

  • RelevanceOne family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Coll 1 antibody
    • Coll1 antibody
    • HH16 antibody
    • Hsema I antibody
    • Hsema III antibody
    • Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A antibody
    • Sema III antibody
    • SEMA1 antibody
    • SEMA1-PEN antibody
    • SEMA3A antibody
    • SEMAD antibody
    • SEMAIII antibody
    • SEMAL antibody
    • Semaphorin 3A precursor antibody
    • Semaphorin III antibody
    • Semaphorin like growth cone guidance homolog of chick collapsin antibody
    • Semaphorin3A antibody
    • SemD antibody
    see all

Anti-Semaphorin 3A antibody images

  • Ab23393 staining human normal ileum. Staining is localized to the cytoplasm.
    Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • Lanes 1 - 2 : Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution
    Lanes 3 - 4 : Anti-Semaphorin 3A antibody (ab25999)

    Lane 1 : Neonatal rat brain
    Lane 2 : Human recombinant Sema3A/Fc chimera (95/125 kDa)
    Lane 3 : Neonatal rat brain
    Lane 4 : Human recombinant Sema3A/Fc chimera (95/125 kDa)


    Predicted band size : 89 kDa
    Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
  • Anti-Semaphorin 3A antibody (ab23393) at 1/1000 dilution + Lysate prepared from Xenopus laevis brain tissue section at 15 µg

    Secondary
    HRP-conjugated goat polyclonal to rabbit IgG at 1/20000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 89 kDa
    Observed band size : 106 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

    This image is a courtesy of Anonymous Abreview

    See Abreview

  • ab23393 staining Semaphorin 3A in 16 µm thick sections of Apteronotus leptorhynchus brain, spinal cord by Immunohistochemistry (Frozen sections).
    Tissue was fixed in 2% paraformaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab23393 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

  • Immunohistochemical analysis of murine developing cerebral cortices, staining Semaphorin 3A with ab23393.

References for Anti-Semaphorin 3A antibody (ab23393)

This product has been referenced in:
  • Ahmed Z  et al. Decorin blocks scarring and cystic cavitation in acute and induces scar dissolution in chronic spinal cord wounds. Neurobiol Dis 64:163-76 (2014). Read more (PubMed: 24384090) »
  • Ranganathan P  et al. Semaphorin 3A inactivation suppresses ischemia-reperfusion-induced inflammation and acute kidney injury. Am J Physiol Renal Physiol 307:F183-94 (2014). Read more (PubMed: 24829504) »

See all 11 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 22 2013

Hello and thank you for contacting us. This antibody have not been tested for sandwich ELISA with a paired antibody, nor has the detection limit of the antibody been tested. I am sorry I could not be more helpful in this case. Please let me know if you...

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Thank you for your reply.
My colleague is currently out of the office and I'm afraid my Spanish is not good enough to reply in this language.
Unfortunately testing customers samples in our lab is not possible.
We appreciate the time you h...

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Ya me han llegado las imágenes, muchas gracias por haber insistido.
Me confirman que este anticuerpo se ha usado en WB con lisados de cerebro de ratón a diluciones 1/250 y 1/1000. La incubación se ha llevado a cabo durante 2 horas a temperatura am...

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Thank you for your call today and for your patience.

I have discussedthe purification kit ab102783with my colleagues, and the minimum amount of antibody that is recommended to use with the kit is 20 ug.Since there are 50 ug per vial of ab2339...

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Thank you for your call today, and I apologize for the trouble with these codes! I have created new codes which will expire on March 1, 2012. I will go through your Abreviews and put these new codes in our internal notes so that the discounts can be ap...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain, spinal cord)
Specification Brain, spinal cord
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Nov 14 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Xenopus laevis Tissue lysate - whole (Brain)
Loading amount 15 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jul 07 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Intervertebral disc/articular cartilage and brain)
Specification Intervertebral disc/articular cartilage and brain
Fixative Paraformaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: 1mg/ml trypsin 20 mins
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 25% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Apr 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (brain endothelial cells)
Specification brain endothelial cells
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% PBS/Triton X100
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Dr. Esperanza Meléndez-Herrera

Verified customer

Submitted Aug 29 2008

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"