Overview

  • Product nameAnti-Semaphorin 3A antibody
    See all Semaphorin 3A primary antibodies
  • Description
    Rabbit polyclonal to Semaphorin 3A
  • Tested applicationsSuitable for: ICC/IF, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide selected from the center region of Human Semaphorin 3A.

  • Positive control
    • Human breast carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab80011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • RelevanceOne family of inhibitory axon guidance molecules is the semaphorins. The semaphorins include secreted, transmembrane, and GPI anchored extracellular molecules that are involved in regulating axon guidance by inhibiting axons from growing toward incorrect targets. Semaphorin 3A (Sema3A) may play a particularly interesting role in limiting axon regeneration since it is expressed in meningeal fibroblasts that invade the injured spinal cord and surround the glial scar. In addition, the Sema3A co receptors, Neuropilin 1 and Plexin A1, are expressed on axons that regenerate up to the injured region, but do not cross this Sema3A containing region. Thus, Sema3A and its co receptors may have important roles in regulating axon guidance during neuronal development and after neuronal injury.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Coll 1 antibody
    • Coll1 antibody
    • HH16 antibody
    • Hsema I antibody
    • Hsema III antibody
    • Sema domain immunoglobulin domain (Ig) short basic domain secreted (semaphorin) 3A antibody
    • Sema III antibody
    • SEMA1 antibody
    • SEMA1-PEN antibody
    • SEMA3A antibody
    • SEMAD antibody
    • SEMAIII antibody
    • SEMAL antibody
    • Semaphorin 3A precursor antibody
    • Semaphorin III antibody
    • Semaphorin like growth cone guidance homolog of chick collapsin antibody
    • Semaphorin3A antibody
    • SemD antibody
    see all

Anti-Semaphorin 3A antibody images

  • ab80011, at a 1/10 dilution, staining Semaphorin 3A in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Peroxidase conjugated secondary antibody was used, followed by DAB staining.
  • ICC/IF image of ab80011 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80011, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Semaphorin 3A antibody (ab80011)

ab80011 has not yet been referenced specifically in any publications.

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