Overview

  • Product name
    Anti-Semaphorin 3B antibody
    See all Semaphorin 3B primary antibodies
  • Description
    Rabbit polyclonal to Semaphorin 3B
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Semaphorin 3B.

    (Peptide available as ab101720.)

  • Positive control
    • This antibody gave a positive signal in Human brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab88489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 83 kDa).

Target

  • Function
    Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
  • Tissue specificity
    Expressed abundantly but differentially in a variety of neural and nonneural tissues.
  • Sequence similarities
    Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
  • Cellular localization
    Secreted. Endoplasmic reticulum. Accumulates in the endoplasmic reticulum.
  • Information by UniProt
  • Database links
  • Alternative names
    • SEM3B_HUMAN antibody
    • Sema A(V) antibody
    • Sema V antibody
    • Sema3b antibody
    • Semaphorin V antibody
    • Semaphorin-3B antibody
    • Semaphorin-V antibody
    see all

Images

  • Anti-Semaphorin 3B antibody (ab88489) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 83 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa,36 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

References

ab88489 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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