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Rabbit polyclonal to Semaphorin 3B
HumanPredicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human Semaphorin 3B.
(Peptide available as ab101720.)
- This antibody gave a positive signal in Human brain tissue lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 83 kDa).
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
Expressed abundantly but differentially in a variety of neural and nonneural tissues.
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain.
Secreted. Endoplasmic reticulum. Accumulates in the endoplasmic reticulum.
Information by UniProt
- SEM3B_HUMAN antibody
- Sema A(V) antibody
- Sema V antibody
Western blot - Anti-Semaphorin 3B antibody (ab88489)
Anti-Semaphorin 3B antibody (ab88489) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466
) at 20 µgSecondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
83 kDaObserved band size:
50 kDa (why is the actual band size different from the predicted?
)Additional bands at:
20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.Exposure time:
The band observed at 50 kDa represents the secreted form of Semaphorin 3B.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"