Anti-Semaphorin 3E antibody (ab112886)
Key features and details
- Goat polyclonal to Semaphorin 3E
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Semaphorin 3E antibody -
Description
Goat polyclonal to Semaphorin 3E -
Host species
Goat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig -
Immunogen
Synthetic peptide:
NGGRYGTTKDYPDD
by a Cysteine residue linker, corresponding to internal sequence amino acids 387-400 of Human Semaphorin 3E (NP_036563.1). -
Positive control
- Human Prostate tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab112886 was purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab112886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Sema domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9723 Human
- Entrez Gene: 20349 Mouse
- Entrez Gene: 296789 Rat
- Omim: 608166 Human
- SwissProt: O15041 Human
- SwissProt: P70275 Mouse
- Unigene: 528721 Human
- Unigene: 134093 Mouse
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Alternative names
- Coll 5 antibody
- Coll5 antibody
- KIAA0331 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (2)
ab112886 has been referenced in 2 publications.
- Wu JH et al. Semaphorin-3E attenuates neointimal formation via suppressing VSMCs migration and proliferation. Cardiovasc Res 113:1763-1775 (2017). PubMed: 29016743
- Mazzotta C et al. Plexin-D1/Semaphorin 3E pathway may contribute to dysregulation of vascular tone control and defective angiogenesis in systemic sclerosis. Arthritis Res Ther 17:221 (2015). IHC-P ; Human . PubMed: 26292963