Anti-Semaphorin 3F antibody (ab39956)


  • Product nameAnti-Semaphorin 3F antibody
    See all Semaphorin 3F primary antibodies
  • Description
    Rabbit polyclonal to Semaphorin 3F
  • Tested applicationsSuitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Semaphorin 3F.

    (Peptide available as ab39955.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Brain (Rat); Brain (Mouse); Brain (Human)



Our Abpromise guarantee covers the use of ab39956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: Use at a concentration of 1 µg/ml. Detects a band of approximately 88,120 kDa (predicted molecular weight: 88 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay play a role in cell motility and cell adhesion.
    • Tissue specificityExpressed abundantly but differentially in a variety of neural and nonneural tissues. There is high expression in mammary gland, kidney, fetal brain, and lung and lower expression in heart and liver.
    • Sequence similaritiesBelongs to the semaphorin family.
      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Sema domain.
    • Developmental stageDetected as early as embryonic day 10.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • SEM3F_HUMAN antibody
      • sema domain immunoglobulin domain Ig short basic domain secreted 3F antibody
      • sema domain immunoglobulin domain Ig short basic domain secreted semaphorin 3F antibody
      • Sema III/F antibody
      • Sema IV antibody
      • SEMA-IV antibody
      • SEMA3F antibody
      • SEMA4 antibody
      • SemaIII/F antibody
      • SemaIV antibody
      • SEMAK antibody
      • Semaphorin IV antibody
      • Semaphorin-3F antibody
      • Semaphorin3F antibody
      • SemaphorinIV antibody
      see all

    Anti-Semaphorin 3F antibody images

    • All lanes : Anti-Semaphorin 3F antibody (ab39956) at 1 µg/ml

      Lane 1 : Human brain tissue lysate - total protein (ab29466)
      Lane 2 : Brain (Rat) Tissue Lysate
      Lane 3 : Brain (Mouse) Tissue Lysate

      Lysates/proteins at 20 µg per lane.

      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 88 kDa
      Observed band size : 100 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 30 kDa,37 kDa,45 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 4 minutes

    References for Anti-Semaphorin 3F antibody (ab39956)

    ab39956 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
    Sample Mouse Cell (Brain sections)
    Specification Brain sections
    Permeabilization No
    Fixative Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jul 24 2014

    Application Western blot
    Loading amount 200000 cells
    Gel Running Conditions Non-reduced Non-Denaturing (Native)
    Sample Mouse Cell lysate - whole cell (Brain tissue)
    Specification Brain tissue
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

    Abcam user community

    Verified customer

    Submitted Mar 13 2014

    Thank you for your reply. My colleague you were in correspondence with is on holiday at the moment. I checked again for you and I have to confirm that ab39955 (Semaphorin 3F peptide) is only a short peptide of 18 amino acids (used a immunogen to genera...

    Read More

    Thank you for contacting us. As you mention, we do have a number of related products available. The SEMA3F products we have in our catalog (such as ab39956) is a rabbit-polyclonal antibody against Semaphorin 3F. It is guaranteed to work with mo...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample Rat Tissue sections (brain sections)
    Specification brain sections
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization No

    Dr. Sophie Pezet

    Verified customer

    Submitted Oct 01 2008