The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Use 0.25µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionMay play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.
Tissue specificityStrongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.
Sequence similaritiesBelongs to the semaphorin family. Contains 1 Ig-like C2-type (immunoglobulin-like) domain. Contains 1 PSI domain. Contains 1 Sema domain.
Cell surface flow cytometric analysis of Semaphorin 4D in human lymphocytes using ab86829 at 0.25 ug/106 cells. Shaded histogram represents cells without antibody; green represents isotype control; red represents ab86829. A Goat anti-mouse IgG PE conjugated secondary was used in this test.
References for Anti-Semaphorin 4D antibody [A8] (ab86829)
This product has been referenced in:
Hall KT et al. Human CD100, a novel leukocyte semaphorin that promotes B-cell aggregation and differentiation. Proc Natl Acad Sci U S A93:11780-5 (1996).
Read more (PubMed: 8876214) »