Overview

  • Product nameAnti-Semenogelin I antibody
    See all Semenogelin I primary antibodies
  • Description
    Rabbit polyclonal to Semenogelin I
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig, Chimpanzee, African Green Monkey
  • Immunogen

    A region within synthetic peptide: ANDHDQSRKS QQYDLNALHK TTKSQRHLGG SQQLLHNKQE GRDHDKSKGH, corresponding to N terminal amino acids 73-122 of Human Semenogelin I

  • Positive control
    • Jurkat cell lysate Human lung tissue Human kidney renal tubule

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • FunctionPredominant protein in semen. It participates in the formation of a gel matrix entrapping the accessory gland secretions and ejaculated spermatozoa. Fragments of semenogelin and/or fragments of the related proteins may contribute to the activation of progressive sperm movements as the gel-forming proteins are fragmented by KLK3/PSA.
    Alpha-inhibin-92 and alpha-inhibin-31, derived from the proteolytic degradation of semenogelin, inhibit the secretion of pituitary follicle-stimulating hormone.
  • Tissue specificitySeminal vesicle.
  • Sequence similaritiesBelongs to the semenogelin family.
  • Post-translational
    modifications
    Transglutaminase substrate.
    Rapidly cleaved after ejaculation by KLK3/PSA, resulting in liquefaction of the semen coagulum and the progressive release of motile spermatozoa.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha-Inhibin-31 antibody
    • Alpha-Inhibin-92 antibody
    • Cancer/testis antigen 103 antibody
    • CT103 antibody
    • dJ172H20.2 (semenogelin I) antibody
    • dJ172H20.2 antibody
    • MGC14719 antibody
    • Semen coagulating protein antibody
    • Semenogelin antibody
    • Semenogelin I antibody
    • SEMG antibody
    • SEMG1 antibody
    • SEMG1_HUMAN antibody
    • Seminal basic protein antibody
    • seminal vesicle secretory protein 5 antibody
    • SgI antibody
    • Svp-1 antibody
    • Svp5 antibody
    • SVPIIA antibody
    • Svs2 antibody
    • SVS2P antibody
    • Svs2p2 antibody
    • Svs5 antibody
    see all

Anti-Semenogelin I antibody images

  • All lanes : Anti-Semenogelin I antibody (ab58692) at 1.25 µg/ml

    Lane 1 : Marker
    Lane 2 : Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Semenogelin I at 4-8µg/ml. Arrows indicate positively labelled alveolar cells. Magnification: 400X.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Semenogelin I with ab58692 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

References for Anti-Semenogelin I antibody (ab58692)

This product has been referenced in:
  • Teirilä L  et al. Proteomic changes of alveolar lining fluid in illnesses associated with exposure to inhaled non-infectious microbial particles. PLoS One 9:e102624 (2014). WB ; Human . Read more (PubMed: 25033447) »

See 1 Publication for this product

Product Wall

Thank you for your reply.

It may indeed be easier to try this experiment with the rabbit monoclonal antibody ab139405. However, the immunogen information is proprietary information. In order to try to help you further could you explain what y...

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Thank you for contacting us and your interest in our products.

This is a very unusual question and I will try my best to help. The anti-Semenogelin I antibody ab58692 has been raised against a synthetic peptide taken from within the region yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"