The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 27 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceCellular senescence, the terminal nondividing state that normal cells enter following completion of their proliferative potential, is the dominant phenotype in hybrids of normal and immortal cells. Fusions of immortal human cell lines with each other have led to their assignment to one of several complementation groups. SEN1 or MORF4 is a gene on chromosome 4 that induces a senescent-like phenotype in cell lines assigned to complementation group B. It is capable of reversing the immortal phenotype of a subset of immortal cells. Overexpression of SEN1 in HeLa cells ultimately caused abnormal nuclear morphology and cell death.