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Senescence Detection Kit (ab65351) is designed to histochemically detect SA-beta-Gal activity in cultured cells and tissue sections, a known characteristic of senescent cells. The SA-beta-Gal is present only in senescent cells and is not found in presenescent, quiescent or immortal cells.
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Senescence is thought to be a tumor suppressive mechanism and an underlying cause of aging. Senescence represents an arrested state in which the cells remain viable, but not stimulated to divide by serum or passage in culture. Senescent cells display increase of cell size, senescence-associated expression of beta-galactosidase (SA-beta-Gal) activity, and altered patterns of gene expression.
|100X Staining Supplement||Red||1 x 1.5ml|
|1X Fixative Solution||NM||1 x 125ml|
|1X Staining Solution||WM||1 x 125ml|
|X-Gal (150 mg, lyophilized)||Green||1 vial|
Our Abpromise guarantee covers the use of ab65351 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution.|
|IHC-Fr||Use at an assay dependent concentration.|
Image obtained from Angelucci S et al; Proteome Sci, 2010 Mar 26;8:18