Overview

  • Product nameAnti-SENP1 antibodySee all SENP1 primary antibodies ...
  • Description
    Rabbit polyclonal to SENP1
  • Tested applicationsWB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from the N-terminus of Human SENP1

  • Positive control
    • Human breast carcinoma tissue. Jurkat cells extract

Properties

Applications

Our Abpromise guarantee covers the use of ab58417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 73 kDa).
IHC-P Use at an assay dependent dilution.
ELISA 1/10000.

Target

  • FunctionProtease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO1, SUMO2 and SUMO3 to their mature forms and deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins. Deconjugates SUMO1 from HIPK2. Deconjugates SUMO1 from HDAC1, which decreases its transcriptional repression activity.
  • Tissue specificityHighly expressed in testis. Expressed at lower levels in thymus, pancreas, spleen, liver, ovary and small intestine.
  • Sequence similaritiesBelongs to the peptidase C48 family.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between cytoplasm and nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • SENP 1 antibody
    • SENP1 antibody
    • SENP1_HUMAN antibody
    • Sentrin specific protease 1 antibody
    • Sentrin-specific protease 1 antibody
    • Sentrin/SUMO specific protease 1 antibody
    • Sentrin/SUMO specific protease antibody
    • Sentrin/SUMO specific protease SENP 1 antibody
    • Sentrin/SUMO specific protease SENP1 antibody
    • Sentrin/SUMO-specific protease SENP1 antibody
    • SUMO1/sentrin specific peptidase 1 antibody
    • SuPr 2 antibody
    • SuPr2 antibody
    see all

Anti-SENP1 antibody images

  • Immunohistochemical analysis of paraffin embedded human breast carcinoma tissue using ab58417 at 1/50 dilution. Samples are treated -/+ blocking peptide.
  • All lanes : Anti-SENP1 antibody (ab58417) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunizing peptide


    Predicted band size : 73 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

References for Anti-SENP1 antibody (ab58417)

This product has been referenced in:
  • Huang W  et al. Triptolide Inhibits the Proliferation of Prostate Cancer Cells and Down-Regulates SUMO-Specific Protease 1 Expression. PLoS One 7:e37693 (2012). WB . Read more (PubMed: 22666381) »
  • Snow JW  et al. Sumoylation Regulates Interaction of FOG1 with C-terminal-binding Protein (CTBP). J Biol Chem 285:28064-75 (2010). WB ; Human . Read more (PubMed: 20587419) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Feb 01 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"